Three Grass Antihypertensive Effect On Shr Hypertension And Antihypertensive Mechanism For Effective Constituents In Soup Of Experimental Research

This study is divided into two parts-the literature review and experimental research.1Literature reviewThe overall research status of Traditional Chinese Medicine (TCM) on hypertention are analysed systematically, including the theoretical research, the clinical research and experimental research. And then make a conclusion of the pathogenesis of hypertention and the depressurization mechanism of herbs of western medicine.2Experimental research2.1ObjectivesTo observe the depressurization mechanism of san cao depressurization decoction (SCD) and explore the relationships between the effective ingredients of SCD and ET-1, NO, ANGI1, and then the relationship between the effective ingredients of SCD and imidazoline receptor.2.2MethodsTake use of macroporous resin adsorption column to separate and extract the effective ingredients of SCD, and choose the optimal effective ingredients on depressurization to take long-term efficacy evaluation.Detect the concentration of AngII and ET-1in the blood of SHR rats treated by the effective ingredients of SCD by ELISA method; Detect the content of NO by the method of nitrate reductase enzymatic; And then take use of western blotting and immunohistochemistry to detect the expression of eNOS、iNOS、 ET-1in rats’tissue; thus to reveal the depressurization mechanism of the effective ingredients of SCD.Set up a group of efaroxan (SCD-95ET+EFA), and treat the group firstly with efaroxan following by the effective ingredients of SCD, thus to explore the correlation of imidazoline receptor (I1R) and the depressurization mechanism of the effective ingredients of SCD.2.3Results2.3.1The depressurization effect of different effective ingredients of SCD on SHR rats.The four groups of effective ingredients of SCD have depressurization effect in different degree. Comparing to the blood pressure before treatment, aqueous extract of SCD plays the role of depressurization4h after treatment (p<0.05); the10%ethamol soluble extract of SCD plays the role of depressurization4h after treatment (p<0.1); the50%ethamol soluble extract of SCD plays the role of depressurization2h after treatment (p<0.1) and still work at4h after the treatment (p<0.05); the95%ethamol soluble extract of SCD plays the role of remarkable depressurization effect2h after treatment (p<0.1) and persistently work at4h after the treatment (p<0.01)There is notable difference of depressurization of95%ethamol soluble extract of SCD and the model group at2h after treatment (p<0.01), and still has difference at4h (p<0.05), There is difference of depressurization of95%ethamol soluble extract of SCD and the aqueous extract of SCD group at2h after treatment (p<0.01), and still has difference at4h (p<0.05). At4h after the treatment,95%ethamol soluble extract of SCD has difference with the10%ethamol soluble extract of SCD group, so as the50%ethamol soluble extract of SCD group (p<0.05)2.3.2The depressurization effect of long-time treatment with95%ethamol soluble extract of SCD on SHR rats.95%ethamol soluble extract of SCD, the optimal effective ingredient of SCD, has an effect of depressurization in the first week (P<0.05), and it still could lower the blood pressure in the third and fourth week (P<0.05). The nifedipine sustained release tablets group, the positive drug group, has a remarkable depressurization effect in the first week (P<0.01),and this effect could lasts to the second week (P<0.05), which still exists in the third and fourth week (P<0.01). Efaroxan group has a notable depressurization effect in the first week(P<0.01), and no statistic significance in the next three weeks. Contrasting with the model group and95%ethamol soluble extract of SCD and nifedipine sustained release tablets group, the qualitity of depressurization has statistically significant difference (P<0.05), and indifference statistics among other groups (P>0.05)2.3.3The effect of the effective ingredients of SCD on NO,ET-1and ANG-II in serum.The antagonism group has the highest content of NO, following by the effective ingredients of SCD and the positive control group, the NO level of the nifedipine group and95%ethamol soluble extract of SCD group arise than the blank group (p<0.05); Comparing to the model group,95%ethamol soluble extract of SCD group has a higher level of NO (p<0.05), there is no difference between the95%ethamol soluble extract of SCD group and nifedipine group (p>0.05)The content of ET-1in scrum of SHR is the highest in antagonism group and there is statistical difference with nifedipine group,95%ethamol soluble extract of SCD group and the blank group (P<0.05).The concentration of ANG-II in rats’ serum has no statistical difference (p>0.05)2.3.4The protein expression of iNOSn eNOS、 ET-1in rats’heart, brain, kidney and aortic arch.The protein expression of iNOS is mainly distributed in vascular endothelial cell(VEC) and smooth muscle cell(SMC). The nifedipine group has a smaller MOD than the model group (p<0.01), the95%ethamol soluble extract of SCD group has a lower protein expression than the model group, which has statistical difference (p<0.05), the protein expression of efaroxan group is more than the blank group with the statistical significance (p<0.05), so as the model group and the blank group.The eNOS is mainly distributed in VEC without remarkable expression in model group and statistical significance comparing to the blank group (p<0.01). The protein expression of nifedipine group is more than the model group (p<0.01).The protein expression of the95%ethamol soluble extract of SCD group is more than the model group with statistical significance (p<0.05)The protein expression of ET-1distributes in VEC. The model group has higher level than the blank group (p<0.01); The efaroxon group has more expressions than the blank group, and with statistical significance (p<0.05)2.4Conclusions2.4.1The effective ingredients of SCD with best depressurization effect is95%ethamol soluble extract of SCD, which has strong effective ingredients on depressurization in it.2.4.2The long-lasting treatment with95%ethamol soluble extract of SCD has a good effect on depressurization which could be used for a long time.2.4.3The95%ethamol soluble extract of SCD could play the function of depressurization by elevating NO and reducing ET-1.2.4.4The depressurization effect of the effective ingredients of SCD may not have relation with ANG-II.2.4.5The95%ethamol soluble extract of SCD could reduce the content of iNOS and elevate the eNOS to regulate and control the formation of NO, and it can also play a synergistic effect on depressurization.2.4.6Efaroxan is an antagonism to the effective ingredients of SCD which indicates there is a correlation between the effective ingredients of SCD and11R.