A Traditional Chinese Medicine Small Compound Screening Method For The Treatment Of The Breast Cystic Hyperplasia

Purpose:Through the ancient and modern literature investigation analysis revealed breast cystic hyperplasia of the etiology and pathogenesis and syndrome differentiation and treatment law:Based on the ancient and modern literature logic and measurement analysis of the characteristics of traditional Chinese medicine establish reflect mammary gland cystic hyperplasia (DBCH) traditional Chinese medicine small compound screening method.Confirm DBCH experimental compound Chinese medicine small intervention of biological effects, and access to the small screen preliminary take prescription, for clinical application and subsequent Chinese herbal medicine new medicine development lay the foundation.Material and method:1. Use modern literature and listed historical analysis of varieties, grasp the syndrome differentiation and treatment DBCH drug laws, and screened DBCH effective treatment of traditional Chinese medicine small compound—Rukangping particles.2. Using the method of pharmacological experiments verify Rukangping particles to the affect of DBCH, Through Rukangping particles implement intervention, testing mice pain threshold value of the physical, chemical and to the influence of the swelling of the ear:assay rats before and after the drug was the second of the nipple diameter changed and histologic examination investigate Rukangping particles in experimental disease of the breast cystic hyperplasia (Guy analgesic experiments in mice, Acetic acid in mice body torsion analgesic experiments, xylene result in auricular swelling of the mice experiment, Rukangping particles to mammary gland hyperplasia of cystic rat models of experiments)Apply the the software of SPSS16.0for windows on processing the result, indicated on the form of x±s. The differences between every two groups are processed by LSD, identify P<0.05as the standar of statistic essence.Results:1. For DBCH discriminate, The authority of the literature have Ganyuqizhi、 Tanshiningju、Xuyu、Chongrenshitiao、Ganyutanning5card.2. The Clinical discriminate of DBCH accord Chongrenshitiao、Tanyuningjie、 Ganyuqizh、Ganyutanning card appear most frequency, respectively in the top lour.3. Guy in mice analgesic experiments, high, medium and low dose group of Rukangping particles mice pain threshold in the30min,60min,90min,120min respectively with the blank group (5.41±0.68),(5.12±1.51),(5.72±1.54)(5.69±1.49) compare, each group can improve the pain threshold in mice, medium dose group (7.43±1.50),(7.55±1.55) and high dose group (8.27±2.93)(6.68±1.14),(7.49±1.70),(7.10±1.15) have statistically significant (P<0.05or P<0.01)4. Acetic acid in mice body torsion of analgesic experiments, high, medium and low dose group of Rukangping particles mice body torsion number (23.00±2.45),(21.90±3.18),(19.10±2.56) compared with blank group (26.70±4.95), each group can reduce the body torsion of the mice,all have statistical significance (P<0.05or P<0O01)5. xylene result in auricular swelling of the mice experiment, high, medium and low dose group of Rukangping particles, the swelling of the ear weight difference in mice (0.00144±0.00066),(0.00104±0.00074),(0.0008±0.00044) compared with blank group (0.00141±0.00065), only high dose group has statistical significance (P<0.05)6. Rukangping particles to mammary gland hyperplasia of cystic rat models of experiments, high, medium and low dose group of Rukangping particles, rats to the determination of the nipple diameter group compared with model (1.28±0.04). medium (1.24±0.04) and high (1.22±0.05) dose group, have statistically significant (P<0.05or P<0.01)7. the second to mammary gland rats histologic examination results high, medium and low dose group of Rukangping particles can different degree of reduce lobular hyperplasia, inhibit gland bubble expansion, duct expansion and ductal epithelial hyperplasia.Conclusion:1. Dialectical standard of the DBCH failed to unity, cause of the academic circles of dialectical chaotic situation.2. The Clinical discriminate of DBCH with different name phenomenon is more serious.3. Rukangping particles can obviously improve the pain threshold in mice, have the role of analgesia.4. Rukangping particles can ease in mice swelling of the ear caused by the xylene, With improving the body immunity.5. Rukangping particles can reduce cystic hyperplasia of mammary gland rat models of nipple second diameter, can alleviate cystic hyperplasia of mammary gland function.