Soft Liver Infusion Of The Experimental Study Of Immune Liver Fibrosis In Rats

Purpose：It is to study the mechanism of Ruangan Chongji on hepatic fibrosis.Method：Ninety-fivewistarratswererandomlydividedintoblankcontrolgroup,model group, colchicine group and each dose of Ruangan Chongji groups, besidesthe blank control group, other groups were injected pig serum in traperitonealto establish autoimmune liver fibrosis model,each rat was given pig serumby0.5ml，twice a week，after five weeks，once a week,injection of10weeks. Atthe sixth week after the model，Ruangan Chongji low-dose group were respectivelyfilled with Ruangan Chongji according to7.5g/kg,5g/kg,2.5g/kg;colchicinegroup were filled with colchicine according to0.1mg/kg; blank control groupand model group were given0.9%normal saline according to10ml/kg, once aday,the end of ten weeks. Observed liver function parameters （AST, ALT, TP,ALB）of rats in Ruangan Chongji group, serum fibrosis markers（HA, LN, PCⅢ, IV-C）of rats in Ruangan Chongji group, as well as liver cell ultrastructure changes,polymerase chain reaction assay the expression of TGF-β₁mRNA.Results：⑴Indicators of liver function: Compared with the control group, the levels ofAST and ALT were significantly elevated in model group, the contents of TP andALB were significantly lower in model group （@P<0.01）. Compared with the modelgroup, the levels of AST and ALT were very significant difference in each doseof Ruangan Chongji groups and colchicine group, the contents of TP and ALB werealso very significant difference in each dose of Ruangan Chongji groups andcolchicine group （*P<0.01）. The levels of AST and ALT were obviously reducd ineach dose of Ruangan Chongji groups, efficacy of Ruangan Chongji groups wassuperior than colchicine group （△P<0.01）. Compared with the colchicine group,the contents of TP and ALB were differently increased in the high and middledose of Ruangan Chongji groups, there was significant difference （△P<0.01or ▽P<0.05）. The contents of TP and ALB in low-dose of Ruangan Chongji group wereno significant difference compared with the colchicine group （P>0.05）.⑵Hepatic fibrosis markers: The contents of HA, LN, IV-C and PCⅢ wassignificantly higher in model group than the normal control group,there wasstatistically significant difference （@P<0.01）. Compared with the modelgroup,the contents of HA, LN, IV-C and PCⅢ have decreased in varying degreesin each dose of the Ruangan Chongji groups and colchicine group, there weresignificant differences （*P<0.01）. The high and middle dose of Ruangan Chongjigroups could descrease the contents of HA, LN, IV-C and PCⅢ,there were obviouslysuperior than the colchicine group （▲P<0.01）. The contents of IV-C and PCⅢin the low dose of Ruangan Chongji group were significantly lower than thecolchicine group （△P<0.05or▲P<0.01）; The contents of HA and LN in the lowdose of Ruangan Chongji group were higher than the colchicine group （▲P<0.01or△P<0.05）. Compared with colchicine group and the low and middle dose ofRuangan Chongji groups,the contents of HA, LN, IV-C and PCⅢ were significantlyreduced in the high dose of Ruangan Chongji group, there was a significantdifference （#P<0.01）.⑶The ultrastructure of liver cells: Liver cell become shrinkage, cell surfacemicrovilli were significantly reduced, intercellular broadens; nucleusshrinkage, and nuclear membrane expansion; The number of the cell organellesremarkably decreased in the cytoplasm; Mitochondrial electronic densityenhancement, cristae disappear, some mitochondria appear empty bubble kindchange; Rough endoplasmic reticulum severe of expansion, the surface of theribosome to fall off, glycogen granules were disappeared, lysosomes less in modelgroup. Hepatocyte morphology is normal,with a large number of mitochondrial,cristae is unclear, rough endoplasmic reticulum less, the ribosome part off onsurface of rough endoplasmic reticulum, glycogen granules increased, scatteredin the cytoplasm, showing a large number of varying sizes dissolved enzymeparticles in colchicine group. Liver cells form in good condition, betweenadjacent cells arranged in neat, rich organelles and mitochondria are smaller, quantity, crest is clearly visible in the high dose of Ruangan Chongji group.Liver cell membrane in clear, nucleus and nuclear membrane is normal, richorganelles and the number of mitochondria is acceptable, smaller, an occasionalcrest, with occasional vacuolar changes, glycogen granules is less in the middleDose of Ruangan Chongji group. Liver cell shrinkage, the number of organellesdecrease in the cytoplasm, a large number of mitochondria are small size, thecrest is not clear in the low dose of Ruangan Chongji group.⑷Molecular markers: Compared with the blank control group, the expression ofTGF-β₁mRNA was increased significantly in model group（@P<0.01）. Compared withmodel group, RI indexes had decreased at varying degrees in the Ruangan Chongjigroups and the colchicine group, there were statistically significant difference（*P<0.01）. The expression of TGF-β₁mRNA was significantly lower in each doseof Ruangan Chongji groups than the colchicine group （△P<0.01）.Conclusion：Ruangan Chongji could effectively improve the liver function andinhibit the TGF-β₁mRNA expression, reduce the level of collagen fibrosis. Itcould mitigate the injury of the supermicro-structure of rats’livers obviously.Stopping hepatic fibrosis progression or deteriorating the pathologic changeof hepatic fibrosis.