Gudou Composite Package Analysis Of Dietary Fibre And The Lipid Metabolic Disorder In Rats And The Impact Of Srebp - 1 C Control

Objective:1. To determine and compare the content of TDF, SDF, IDF in whole grain-bean package and whole grain corn. Determine the best process of DF extraction, and measure the physical and chemical property of extracted DF.2. To observe and compare the impacts of whole grain-bean package, DF extracted from whole grain-bean package, and DF from whole grain corn on the blood lipids and FAS activity in high-fat, and high-cholesterol feeding induced Dyslipidemia Rats, and observe its effect on regulation of SREBP-1c mRNA expression in rat liver.Methods:1. Use Enzyme-chemical method to extract total dietary fiber of whole grain-bean package and whole grain corn, and use Enzyme-weight method to determine dietary fiber.2.50SD rats of clean grade feeding adaptation for one week. Cut the tail and collect serum. Analysis lipids(TC, TG, HDL-C, FBG) level. According to TC levels,50SD rats were randomly assigned into normal control group, hyperlipidemia model group, whole grain-bean package group, whole grain-bean package dietary fiber group, and whole grain corn dietary fiber group. According to AIN-93M animal Synthesized feed composition, make the normal control group feed. Based on this, make hyperlipidemia model feed, whole grain-bean package group, whole grain-bean package DF group, and whole grain corn DF group by adding10%saturated fatty acid and1.5%cholesterol.3. Feed continuously for eight weeks, and measure the TC, TG, HDL-C, FBG levels of all groups. Get one leaf of liver from killed rats, use10%methanal to fix, HE dyeing, and observe hepatic steatosis situation under microscope.4. To use ELISA methods to measure the FAS activity in rat liver.5. To use RT-PCT method to detect experimental rat liver SREBP-1c mRNA expression.Results:1.100g whole grain-bean package extract14.5g dietary fiber on average.100g whole grain corn extract5g dietary fiber. 2.100g whole grain-bean package contain TDF15.3g, IDF13.97g, SDF1.33g.100g whole grain corn contain TDF9.1g, IDF8.10g, SDF0.996g.3. Whole grain-bean package DF and whole grain corn differ in expansibility:10.19ml/g and8.1ml/g; in water-holding capacity:8.83g/g and7.7g/g; in Unsaturated fat absorption ability:1.64g/g and1.05g/g; in Saturated fat absorption ability:1.89g/g and1.2g/g.4. Results from animal research shows:compared with normal control group,TC,TG, FBG, LDL-C levels of hyperlipidemia model group were significantly decreased (p<0.05).Compared with hyperlipidemia model group, TC,TG, FBG,, LDL-C levels of whole grain-bean package group, whole grain-bean package DF group, whole grain corn DF group were significantly decreased (p<0.05); HDL-C levels in whole grain-bean package group, whole grain-bean package DF group, whole grain corn DF group are higher than hyperlipidemia model group.5. HE dyeing results show whole grain-bean package, whole grain-bean package DF, whole grain corn DF can relieve the degree of hepatic steatosis situation.6. FAS activity in rat liver of whole grain-bean package group, whole grain-bean package DF group, whole grain corn DF group are much lower than hyperlipidemia model group. And SREBP-1c mRNA expression decreased significantly.Conclusions:1. Whole grain-bean package contain abundant dietary fiber,about1.7times than whole grain corn.the yields of whole grain-bean package DF is5times than whole grain corn DF. The reason maybe whole grain corn contain lots of starch which could not easily removed.2. DF from Enzyme-chemical method have better physical and chemical property, a lager yields, and higher purity.3. Whole grain-bean package DF can improve blood lipids levels of Dyslipidemia Rats.4. Whole grain-bean package DF can decrease FAS activity and SREBP-1c mRNA expression.