Microfluidic Design And Simulation For Cell Detection

Biological cell is the basic unit for constituting functional structure in various organisms. On the other hand, the cell is also the basic part for diseases form. The significance obviously is there for relationship between cellular and molecular biological research, early diagnosis of the disease research by use surface stress theory.The biosensor coming from cell study has been a hot research field. The many detection approaches mainly focus on fluorescent methods, chemiluminescence test, acoustic wave sensors, mass spectrometry and surface Plasmon resonance sensors. The labeling process of cellular and molecular is harmful for functional and stability of cells. Furthermore, the optical instrument is bulky and not easy integration package. There are many inconveniences to the real-time detection of cells.As a key tool for loading and cleaning of the analytic, microfluidic technology is an important part for integrated biosensor, miniaturized system. The microfluidic chip cell research is closer to the true state of the cells in the body. In addition, the cell manipulate is easy process, integration and other advantages based on the microfluidic chip. The further study is a general trend.In this paper, based on the common and typical structural design of the microfluidic, a novel microfluidic is proposed on the foundation of actual experimental studies. The novel microfluidic structure has the following characteristics:uniform fluid velocity of a detection region, the small shear force in test microchamber, simple design for microarray structure. The advantage of this design is that a practical approach is proposed for the tiny signal extraction. On the other hand, the design is contribute to reducing the fluid damage to cells through decreasing the shearing force generated by the micro-reaction chamber.The finite element method was employed to optimize the fluid microenvironment. Comparing three typical microfluidic structures with our novel design, the microfluidic array model was ultimately determined. Moreover, on the basis of a microfluidic array structure optimization of the critical dimensions was obtained.On the basis of the micromachining process, the subject program was proposed. As a good biocompatibility of this material and a relatively small Young’s modulus of PDMS (polydimethylsiloxane), it is the suitable material for processing. Meanwhile, a viable microfluidic arrays process was proposed in this paper.