The Microfluidic Design For Cell Culture And Its Application In Biological Sensing Detection

The microfluidic chip has a significant impact on the research of cell culture,testing and analysis. And, the detecting platform combined with biosensortechnology provides a more appropriate approach for the research of cellconcentration distribution and physiological state. Microfluidic system can notonly provide a suitable cell culture environment to achieve cell culture andmanipulation, but also can be combined with the biosensor to build ahigh-sensitivity, high accuracy, good biological compatibility and portability cellresearch platform. All of these provide practical work experience for cell culture,detection, analysis and bio-chip integration.In the paper, a zero-flow microfluidic design is proposed, which can be usedfor long-term cell culture and detection, especially for a lab-on-chip integratedwith a biosensor. The Finite Element Method (FEM) was employed to predictthe fluid transport properties for a minimum fluid flow disturbance. And, cellculture experiment, cell concentration and state detection experiments wereaccomplished to verify its application in biosensing detection. The main contentsare as the follow aspects: 1. Based on the theoretical analysis of microfluidics, the preliminary design ofthe system structure was completed. Then, some commonly used microflidicstructures were analysed systematically to optimize and prove the designedstructure. The Finite Element simulation was employed to identify the specificstructure size, initial conditions and the reasonableness of the microfluidicstructure. The results show that, under the set size and initial conditions, thisdesign provides a continuous flow environment, with a relatively stable and lowshear stress atmosphere, similar to a zero-flow environment. It can be used inthe field of cell culture and biological sensing and improve detection accuracyand resolution.2. The processing technology of microfluidic system was confirmed in thepaper. Then, based on the micro machining technology, the system structurepreparation was completed. Cell culture experiment platform and cell sensordetection experiment platform (a PDMS micro-membrane capacitive surfacestress biosensor) were set up. Main processing process is divided into:preparation stage, cationic membrane processing stage, covering plateprocessing stage and stage of platform.3. Finally, staphylococcus aureus was employed as the bioanalyte to test themicrofluidic system. Based on the measuring experiment platform, the cellculture experiment, cell concentration measurement experiment and cell statedetection experiment were carried out. Results show that the designedmicrofluidics provides a micro environment of approximate zero flow zero shear-force. It can not only simulate the in vivo environment of cell culture for along time, and provides biological sensor to detect interference detection with astable environment, greatly improve the biological sensor detection range andaccuracy.