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The Response To Earthquake Of Microorganism In The No.10Spring Of Urumqi

Posted on:2014-12-02Degree:MasterType:Thesis
Country:ChinaCandidate:J XueFull Text:PDF
GTID:2250330401483200Subject:Biochemistry and Molecular Biology
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Objective: This study aimed to investigate the bacterial community structure and diversity in the No.10spring Urumuqi durning the24solar terms, looking for reflecting characteristics bacterial communitiesdurning different earthquake earthquakes; analysis the structure of archaeal community composition in thespring. Methods: Terminal restriction fragment length polymorphism analysis (T-RFLP) is used todiscuss the structure and diversity of bacteria and archaea community, study the correlation betweenbacterial communities and environment factors by canonical correlation analysis (CCA); discussing theearthquake reflecting characteristics and response mode of bacteria communities under different magnitudeand differerent epicentral distance. Results:①In the24solar terms, the bacteria diversity fluctuationobviously; the community contributed by11phylums, Actinobacteria, Bacteroides, Firmicutes,Fusobacterium and Proteobacteria as the dominant communities take75%in the hole community, somehydrogeochemical parameters such as electrical conductivity, fluoro, methane, sulfide, etc. have asignificant effect to bacteria diversity; Nitrogen, sulfides, conductivity, and the total gas do great effects onthe dominant T-RFs, Radon, argon, methane, fluorine and helium do great effects on the specialT-RFs;②B acteria in No.10springs have a good earthquake reflecting characteristics to Near-fieldearthquakes and Far-field earthquakes Ms≥3.0; bacteria diversity and magnitude can be positivelycorrelated, earthquakes happens durning Shannon index’s increasing;③conductivity, fluoride, water,mercury, methane and radon distribution and some other hydration parameters do great effects on thebacterial community, relationships between some dominant classes, such as Actinobacteria, Flavobacteria,Bacilli, Deltaproteobacteria Gammaproteobacteria and the hydration parameters are Suppression; Bacteriabelongs to Fibrobacteres, Tenericutes and Spirochaetes etc. only appeared before-or after-earthquakes,suspect their appearance can relate to the earth crust;④Aecheal belong to Euryarchaeota andThaumarchaeota, Methanomicrobia is the dominant class, methanogens play an important role in thecarbon cycle. Conclusion: Bacteria community and diversity can related to hydrogeochemical parameters,earth crust can increase the diversity of bacterial community, some bacteria group are sensitive toearthquakes; The aecheal community structure is simple, rich in methanogenic archaea, No.10Spring has apotential value of development and untilization of microbial resources.
Keywords/Search Tags:Urumqi No.10, Spring, Microorganism, T-RFLP, diversity, earthquake reflectingcharacteristics
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