Miolecular Diversity Of Atmospheric Microbiology In Spring In Three Function Regions Of Nanjing City

The air microorganism plays an important role in the global ecosystem of the city, which has the extremely important ecosystem function. In the city ecosystem, the air microorganism affects the city air quality. It was closely related with the human body health. The high energy’s falling dust in Nanjing city not only reduces atmospheric visibility, but also has the serious corrosion to the urban architecture. The microorganism can obtain the nutrients in the air falling dust, and then multiplies massively. Pathogenic microorganism can spread disease through the falling dust migration. The global warming already became the focal point which the people pay more attention to. The biology is playing the noticeable role in the carbon dioxide fixing aspect.In this study, a PCR-based cloning and sequencing approach was used to investigate the molecular diversity and community structure of microorganism in the in Nanjing City by using the traditional cultural technology and analyzing the diversity of16S rDNA,18S rDNA. We also had been investigated the molecular diversity and community structure of Carbon dioxide fixing microbes with the Carbon cycle.In order to understand the community structure of bacteria microbes in atmospheric, three16S rDNA libraries were constructed (NAU, NBG, XWG).16S rDNA were amplified with universal primers of bacteria,305the PCR products were digested with restriction endoenzyme Hha I and Xsp I respectively and analyzed by RFLP analysis. The results showed that the liquid microorganism sampling instrument more suitable to carry on gathering to the air bacterium. All statistical results showed that microbial diversity of three16S rDNA clone libraries was rich very much.16S rDNA sequencing indicated that the main bacteria genus such as Bacillus, Pseudomonas, Micrococcus are all in the system.In order to understand the community structure of fungus in falling in atmospheric, three18S rDNA libraries were constructed (NAU, NBG, XWG). The results showed that Nanjing spring’s air fungus’s quantity may reach104CFU/m. Phylogenetic analysis suggested that the fungus were Alternaria, Penicillium, Aspergillus, Sistotrema, which was consistent with many other studies.To test microbial autotrophic CO2fixation plays an important role in systems of air several samples were taken and used to investigate the distribution and phylogenetic diversity of ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) large subunit. The cbbL genes were found to be the closest associated Coccomyxa and Hydrogenophaga. They might also play important roles in the carbon dioxide fixing aspect.