Preparation Of Blueberry Anthocyanin And Its Products

Blueberry anthocyanin has varies physiological activities, such as promote and activate rhodophanesynthesis, increase visual acuity, improve circulation, anti-ulcer, anti-inflammatory, enhance immunity andcariac function, anti-cardiovascular disease, anti-aging, anti-cancer and anti-mutation and so on. Therefore,blueberry, known as “the world’s third-generation fruit” after the apples and oranges, has great economicvalue and broad development prospects.Based on the research of cultivate blueberry, especially on blueberry anthocyanin extraction,purification and blueberry fruit wine and beverage, this paper is aimed to provide reference for thedevelopment and utilization of blueberry natural pigments, and provide theoretical basis for blueberrymulti-directional and further study. The work of the study are as follows:The best blueberry anthocyanin optimum extraction Process: the optimal acetic acid concentration,extraction time, extraction temperature, and the ratio between solid and liquid are20%,30℃,60min and1:10, respectively, under which the yield of anthocyanin is92.8%. The influence of concentration of aceticacid on the content of anthocyanidin is greater.Blueberry anthocyanin is purified by AB-8and SP-207macroporous resin. By using AB-8macroporous resin to purify blueberry anthocyanins, the macroporous resin extract size to volume ratio is1:1, and the adsorption capacity of anthocyanin is0.18mg/ml. The crude anthocyanin is eluted by twocolumn volume of40%ethanol aqueous solution. As a result, the blueberry anthocyanins purity is42.8%,and the yield of pure discount is56.2%. By using SP-207macroporous resin to purify blueberryanthocyanins, the macroporous resin extract size to volume ratio is0.2：1, and the adsorption capacity ofanthocyanin is0.9mg/mL. The crude anthocyanin is eluted by two column volume of40%ethanol aqueoussolution. As a result, the blueberry anthocyanins purity is52.2%, and the yield of pure discount is57.6%.The SP207macroporous resin adsorption capacity of anthocyanin is5times than AB-8macroporous resin,and its purification effect is much better than AB-8macroporous resin.The best blueberry wine fermentation temperature is25℃.The main fermentation time is7~10d, andthe secondary fermentation time is10~15d at the temperature of25℃, under which blueberry wine smellsmellow pure and refreshing;blueberry beverage best formula: fruit juice content15%, the best sugar dosage 10%, acid dosage0.15%. The beverage is acid palatability of sweet, and has unique flavor.