Molecularly Imprinted Polymer For Recognition Of Protein By Metal Coordinate Interaction

In this paper, the inclusion complex formed between poly(vinyl alcohol)(PVA), a water-soluble polymer, and y-cyclodextrin (y-CD) is prepared under80℃at first. Then the inclusion complex is modified by acryloyl chloride to obtain assistant recognition polymer chains (ARPCs). These ARPCs had numerous double bond groups on the inclusion complex chains that could reach a three-dimensional distribution with the template. FT-IR spectra shows that the double bonds have been successfully introduced into the inclusion complex chains as expected.Based on metal coordinate interaction, the molecularly imprinted polymer was prepared by using acryl amide as the functional monomer, N,N’-methylene bisacrylamide as the cross linker, modified inclusion complex as the ARPCs, BSA as the template, and APS and NaSO3as the initiator. To test the selectivity and adsorption capacity of imprinted polymers, a series of adsorption studies were conducted. Scanning electronic microscope (SEM) was used to investigate the morphology of MIPs. Adsorption dynamic studies showed that the adsorption capacity of MIPs with ARPCs and metal ions was obviously improved. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) results showed that BSA washed out with0.15mol·L-1KCl solution should be the non-specific adsorption proteins, while BSA washed out with0.50mol·L-1KC1solution and2.00mol·L-1KCl solution should be the specific proteins. The introduction of ARPCs and metal ions to MIPs improved significantly the specific adsorption to BSA. The separation factor to mixture protein is about24.718.