Microarray Analysis Of Hepatic Steatosisrelated Genes

Non-alcoholic fatty liver disease is a very common liver disease. In recent years, because of the improvement of people’s living standard and changes of people’s life-style the morbidity of fatty liver disease became higher and higher especially in developed countries and areas. Non-alcoholic fatty liver will develop into liver cirrhosis and even liver cancer if it continues deterioration. The development of NAFLD usually is divided into two phases. The first phase is that fat accumulates in liver which becomes hepatic steatosis. The second phase is the development of nonalcoholic steatohepatitis(NASH) which is caused by oxidative stress which is because the long time exposure to fat.In this article we used gene microarrays to study the mechanism of hepatic triglyceride accumulation in three different models. These three models is made by post-fast high carbohydrate diet, fasting for24hours and rapamycin treatment.We analyzed the microarray data by using bioinfomatic technique and came to some conclusions that showed blow.1. Under hepatic steatosis condition,glucose metabolism, fatty acid biosynthesis, lipid and ion transport and cell cycle all showed some changes. In the same time the transcription level of many genes that have binding activity or transporter activity or oxidoreductase activity changed too.2. Hepatic steatosis is regulated by pathways that related to glucose and lipid metabolism, oxidative stress and complement and coagulation cascades.3. APOA4、CGREF1、SCD3may play an important role in hepatic steatosis and deserve further study.