Bioinformatics Analysis Of Glutamine Supplementation On Signaling Pathways In HepG2Cells

Glutamine is a non essential amino acid but it is essential for cell proliferation and its presence is required for the smooth process of cell signaling pathways. The mechanisms of differences in protein expression changes and functional annotation during re-supplemented glutamine it is still not fully understood. To identify the molecular pathways associated with glutamine signaling pathway, we performed bioinformatics analysis of glutamine deprivation and re-supplemented glutamine in HepG2cells using iTRAQ approach (isobaric tags for relative and absolute quantitation). The samples of cells were harvested for extract and cleaved with trypsin and the resulting peptides were labeled with iTRAQ reagents. Labeled peptides separated by strong cation exchange and reversed phase LC and analyzed by MALDI-TOF/TOF MS. Proteins identification were performed by using Mascot search engine (Matrix Science, London, UK; version2.3.02) against database containing the related sequences.The results of this study found10unique proteins were identified differentially expressed in HepG2cells. Proteins found to be associated with their functions on GFPO, TRSB, EPC, LTM, AATM, and SMBTC, even found significantly up to down regulated proteins ALDH8A1and ADH1C during both treatments. We conclude that the up-regulated proteins induced by glutamine deprivation because over-expression of the proteins due to metabolic stress. Instead, re-supplemented glutamine, the cells nutritional needs can be distributed and fulfilled, so that the expression of proteins associated with the pathways going down again. In summary, our finding provided a comprehensive protein profiles relevant to the signaling pathways of glutamine deprivation and re-supplemented glutamine in HepG2cells.