Ionizing Radiation Inducing Immune Reaction Related Cell Factors Expression

Help T cell in the immune response plays the role of the intermediate:activated to generate a direct immune response of the immune cells can proliferate. Help T cell react differently to cytokine stimulation, the formation of the dynamic balance of the body a variety of help T cell subsets. Under normal circumstances, the body in a variety of help T cell subsets in a state of relative balance dysfunction in the body, often showing the balance to be biased either party. Once the equilibrium between cell subsets was broken, it may result in cytokine secretion in vivo dynamic equilibrium is destroyed, which leads to the emergence and development of many diseases. The body of the biological effects of different doses of ionizing radiation is different from.In this study, the role of different doses of ionizing radiation is observed Thl, Th2, Th3/Trl landmark changes in the amount of cytokine secretion, explore the relationship between cell balance and radiation dose.In this study, detected by ELISA at different doses of irradiation, while the establishment of the sham control group, total body irradiation mice after16h, spleen and thymus Thl-type cytokines IFN-y, Th2-type cytokines IL-10, Th3/Trl cytokine TGF-β1and IL-27secretion volume changes. And external irradiation DC/TLC co-culture system in IFN-y and IL-27, TGF-β1secretion amount of change.Experimental results show that, Th1-type cytokines in spleen cells to secrete IFN-y in the low-dose irradiation and the secretion of the sham-irrad group did not change significantly. The amount of irradiation of the thymus cells to secrete IFN-y were higher than those in the sham-irrad group. The DC/TLC co-culture system dose0.075Gy,24h and48h after irradiation, IFN-y secretion was significantly higher than sham-irrad group (P<0.05).2.000Gy irradiation after6to24h significantly lower than those in the sham-irrad group (P<0.01). DC/TLC co-culture system IFN-γ secretion in the radiation dose0.200Gy, higher than that in the sham-irrad group (P<0.05-0.01)Th2cytokines in mouse spleen cells the0.100-1.OOOGy after irradiation the secretion of IL-10was significantly lower than the sham-irrad group (P<0.05), thymus in the IL-10secretion in the the0.100-6.OOOGy after irradiation higher than that in the sham-irrad group (P<0.01).Th3type cytokines in mouse spleen cells to secrete TGF-β1in2.OOOGy and6.OOOGy group in the high-dose group was significantly higher than that in the sham-irrad group(P <0.01). The thymus cells secrete TGF-β1low doses significantly lower than the sham-irrad group (P<0.01),2.000-6.OOOGy group in the high-dose group was significantly higher than that in the sham-irrad group (P<0.01). DC/TLC co-culture system dose of0.075Gy TGF-β1schedule secretion were lower than the sham-irrad group. The radiation dose was2.000Gy,12h,24h and48h TGF-β1secretion was significantly higher than that in the sham-irrad group,72hours after irradiation was significantly lower than the sham-irrad group (P<0.05-0.01). DC/TLC co-culture system, the radiation dose for0.075,0.100and0.200Gy, when the secretion of TGF-01significantly lower than those in the sham-irrad group (P<0.01), the radiation dose for the2.000-6.000Gy when secretion of TGF-01significantly higher than that in the sham-irrad group (P <0.05-0.01)The amount of irradiation of the thymus cells to secrete IL-27were higher than those in the sham-irrad group. DC/TLC co-culture system after0.075Gy irradiation12to72hours, IL-27secretion was significantly higher than that in the sham-irrad group (P <0.05-0.01).2.000Gy irradiation after48to72h significantly higher than sham-irrad group (P<0.01). DC/TLC co-culture system IL-27secretion for of0.200,1.000and6.000Gy when irradiation dose higher than that in the sham-irrad group (P<0.05-0.01) IL-27secretion is also in2.000Gy after irradiation was significantly higher than that in the sham-irrad group (P<0.01), other experimental group and the sham-irrad group did not change significantly.