Study On Advanced Observation Technology Of Cyanobacteria In Tai Lake Based On Cell Physiology Of Algae And Its Applications

Based on algae cell growth and physiological characters, a novel indoor observation on Cyanobacteria growth from Tai Lake and its applications were investigated in this thesis.An indoor cultivation of Tai Lake water was established, it showed that there were a close correlation among the parameters of pH, carbon dioxide reaction rate (CRR), dissolved oxygen (DO) and Chlorophyll a (Chl a). The experimental results were as follows:①during the formation process of algal bloom, the pH, Chlorophyll a and DO had the same changing trend, while carbon dioxide reaction rate (CRR) with the opposite trend;②about the different seasons in2010,2011, the study of Chlorophyll a and CRR variation showed that during the late June to mid-September was the peak of out breaking of algal bloom. And the degree of eutrophication in2010was higher than in2011;③for four consecutive years in Spring at the same location in Tai Lake, the study of indoor simulated cultivation showed that, compared to2009,2010,2011, the year of2008was easier formation of algal bloom. That meant the water quality in spring became worse year after year. In addition, the parameters between CRR and Chlorophyll a were inversely related by the method of mathematical models. We also used the changes of CRR and Chlorophyll a to characterize the process of algal bloom formation.Microbial community structure changes during the formation of algal bloom in Tai Lake using PCR-DGGE method, the results showed that, in the early time of algal cultivation, the algal belonged to Microcystis genus (Microcystis), while in the late time of cultivation, the algal belonged to Synechococcus, Cyanobium of Cyanophyta (Cyanophyta). Throughout the Cyanobacteria growth process, the Tai Lake water may had been contaminated by Farm animal feces.