| In recent years, the soil environment pollution problems are worsening. With long-term application of chemical fertilizers, pesticides and car exhaust emissions and other factors, the tea garden soil is affected to varying degrees of lead, copper and other heavy metal pollution, which ultimately create a potential threat to human health. Microbiological adsorption heavy metal has the advantage of wide sources, high efficiency and easy operation, which has been widely used in the treatment of soil heavy metal pollution.10different anti-Cu fungi were screened from high copper in soil samples. One kind of fungi named A4had the highest abilities of resistance and enriching copper. Cu-rich conditions by single factor experiments and orthogonal test were researched. Results showed that the culture time were the most important factor of enrichment, followed by the initial copper ion concentration and inoculum size, and the last was the rotate speed. The optimal conditions for strains enrichment of copper ion were72h of incubation time,1%of the initial inoculum size,100mg/L of copper ion mass concentration,130r/min of speed. According to the colony morphology, microstructure, scanning electron microscopic features as well as the DNA sequencing of the tested strains, it was identified as Aspergillus fumigates.The tea garden soil heavy metal content in Chang’an teaching practice base were detected and analyzed by GB15618-1995, NY5199-2002, NY5020-2001three criteria. The results showed that the important element of tea garden soil heavy metal pollution was Cd, followed by As. According to the evaluation of the integrated pollution index, the tea garden soil environmental quality exceeded the warning level. Aspergillus fumigates. was applied to enrich copper in tea garden soil. The results showed that the strain of Aspergillus fumigates had certain adsorption to the tea garden soil of copper, which providing a candidate bacteria resource of adsorption copper in the tea garden soil. |
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