Study On Microorganism Distribution And Removing Correlation In Different Types Of Constructed Wetlands

In the constructed wetland treatment system, plants, substrate, and microorganismsinvolved in a series of complex physical, chemical and biological reaction，wheremicroorganisms are play an important role for the degradation of pollutants, and theyare the major contributor to sewage treatment effect. They are interaction andinterdependence with the environment. Therefore, the microbial diversity and thedistribution of changes in the constructed wetlands have significance to the purificationof polluted river water. In this paper, we analyzed the number and distribution law of themicroorganisms in pilot models and small scale constructed wetlands by traditionalculture methods, and study the correlation between number of microorganisms andpollutants removal efficiency in the small scale model. Major results are shown asfollows:Along the water-flow direction, take the surface flow constructed wetlands forexample, in1#, The number of bacteria, actinomycetes, ammonization have asignificant negative correlation with the increase of the distance （p<0.05）.In2#, Thenumber of bacteria, has a greatly significant negative correlation with the increase of thedistance （p<0.01）, The number of fungi, actinomycetes, ammonization have asignificant negative correlation with the increase of the distance （p<0.05）.Along the vertical space, take the Subsurface Flow Constructed Wetlands forexample, in the constructed wetland system4#,the upper number and lower number ofbacteria、actinomycetes were greatly significant difference （t<0.01）. The upper numberand lower number of fungi in constructed wetland system3#and4#were significantdifference （t<0.05）. The upper number and lower number of mamoniyfing bacteria inconstructed wetland system3#were greatly significant difference （t<0.01）, and in theconstructed wetland system4#,the upper number and lower number of mamoniyfingbacteria were significant difference （t<0.05）.In5#、6#Composite Flow ConstructedWetlands，the upper number and lower number of denitrifying bacteria were greatlysignificant difference （t<0.01）.From the activity of microorganisms, take the Composite Flow ConstructedWetlands. Through the T-test, in both Wetlands, nitrification intensity were greatlysignificant difference between the upper substrate and the lower substrate （t<0.01）.Denitrification intensity was greatly significant difference between the upper substrateand the lower substrate （t<0.01） in5#. The relationship between number and activity ofmicroorganisms indicated: there was positive correlation between number ofnitrification bacteria and nitrification, but the difference was not significant, at the same time, there was significant positive correlation between number of denitrifying bacteriaand denitrification in6#（p<0.01）. However，in5#, it was no significant.The relationship between number of microorganisms and removal rates indicated:there were positive significant correlation between bacteria number and removal rates ofCOD_Mn, TN （p<0.05）, but there was no significant correlation between bacteria numberand removal rates of TP （p>0.05）. There was no significant correlation between fungi,actinomycetes number and removal rates of COD_Mn, TN, TP. mamoniyfing,nitrificationbacteria number and removal rates of TN were no significant correlation, butdenitrifying bacteria number and removal rates of TN was positive significantcorrelation（p<0.05）. mamoniyfing bacteria, nitrification bacteria umber and removalrates of NH₃-N were greatly positive significant correlation（p<0.01）.