| Effect of different pretreatment methods of food waste and sluge on fermentative hydrogen and methane production in batch test was studied in this paper. Properties of food waste and initial microbial community structure were investigated for the mechanism analysis.The best performance was gained when food waste and sluge were both pretreated by70℃/30min in the range of50℃～100℃with32.23%hydrogen content and53.55mL-gVS "’hydrogen production rate,7.39times and30.88times higher than the control, respectively. The fermentative pathway was shift from ethanol fermentation to acetate and butyrate fermentation with the total mass concentration of acetate and butyrate reached2300mg·L-1.Methane production rate was745.36mL·gVS-1. There was no significant difference in gas production and VFAs when the temperature of pretreatment was50℃and60℃compared with the control.Futher more, to obtain higher net energy yield, acid-pretreament and alkali-pretreatment with lower energy consumption were investigated. No significant difference in gas production and VFAs was observed in alkali-pretreatment, while with acid-pretreament the highest hydrogen and methane production rate were gained with40.46mL·gVS-1and745.36mL·gVS-1, respectively, higher than that of70℃heat-pretreatment.Using electron microscopy and PCR-DGGE technology, significant differences of dominant strains were observed compared to the control. The dominant strains were the same within three different pretreatment methods and the lowest Simpson index was observed within acid-pretreament indicating the highest dominance. |
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