Synthesis And The Usage Of A New Prop-Molecular Imprinting Ploymers Withβ-Cyclodextrin (Derivatived With2,4,6-trinitrophenol)/Silica Gel As Support

Propachlor is one of the most widely used acylanilide herbicides which is used for the preemergence control of many annual grasses and certain broad eaved weeds. Its toxicity was stabile to natural decomposition and persistence in the environment. The widespread use of propachlor has lead to increased food, soil, and water pollution by its residues. It is necessary to develop analytic methods for determining its residues in environment at low concentration.Currently, a series of methods such as solid phase extraction, soxhlet extraction, liquid-liquid extraction, thin layer chromatography and column chromatography are used to pre-treat herbicide and its residue. However, these methods have several inherent limitations such as time-consuming, consumption of organic solvents and requirement of costly equipments for sample pre-treatment.Among many processes proposed and/or being developed for the treatment of the organic contaminants, molecule imprinting technique has attracted the attention as separation media solid-phase extraction due to their selective molecular recognition properties. However, MIPs prepared by some conventional methods have disadvantages such as irregular shape and deeply embedded recognition sites, which may cause poor site accessibility for the target molecule. To solve these problems, the surface molecular imprinting technique has been taken to improve the mass transfer between recognition sites and target molecules by designing the molecular recognition sites on the surfaces of imprinted materials.In this study, we synthesized the MIPs using Prop as the template molecule. The major topics in the thesis include: 1. An artificial receptor was synthesized by the method of molecularly imprinted polymer (MIP1). Trinitrophenol-derivatized β-cyclodextrin bonded silica (PCDS) was used as supporting matrix. The preparation of molecularly imprinted polymers (MIPs) for Propachlor (a-chloro-N-isopropyl-acetanilide) was performed on the surfaces of the modified silica gel using propachlor as the template, acrylamide (AA) as the functional monomers, ethylene glycol dimethacrylate (EDMA) as the cross-linker and2,2-azo-bis-isobutyronitrile (AIBN) as the initiator. Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR) were used to characterize the prepared MIPs.2. The results of the binding characteristics of the imprinted polymer and the Langumir analysis show that molecularly imprinted polymer has good retention effects on template.3. Solid phase extraction (SPE) with the Prop-MIPs as a adsorbent was investigated. The optimum loading, washing and eluting conditions for prop molecularly imprinted polymer-solid phase extraction were established. After mixing sample was loaded in solid-phase extraction column, Prop, Acet, Napr and Prom can be separated using different composed eluting solvent cooperated with the step by step washing method.4. The optimized Prop-MISPE procedure was developed to enrich and clean-up the Prop residue in water, soils and rice. Finally, concentrations of Prop in the samples were analyzed by HPLC-UVD. The average spiked recoveries of Prop in water, soil and rice were84.4～87.4%(RSD,3.39～6.06%),86.9～97.1%(RSD,3.52～5.82%) and82.5～94.2%(RSD,1.58～6.34%) respectively. Overall, our study provides a sensitive and cost-effective method for accurate determination of Prop residues in water, soils and plants.