Spectrophotometry Study And Application Of Charge Transfer Reation Of Pharmaceutical Analysis And Interaction Between Drugs And Bovine Serum Albumin

Cephalosporins are a class of commonly antibiotics in the world, and the clinical application has always accounted for the first. Piperlonguminine (PLG) is a pepper alkali derivative and has significant lipid-lowering effect which is extracted from Long pepper. But so far there is little documents to accurate determine the concentration of PLG. Charge transfer spectrophotometry is brief, fast and exact analysis method which is widely used in pharmaceutical analysis.So it has good prospects. The drug analysis not only includes the content determination of drugs but also the studies on pharmacological and efficacy of the drugs. Then study on the interaction between drugs and serum proteins in the molecular level can not only contribute to understanding the process of drug transportation and metabolism but also provide more valuable information to design and development new drugs. Using cephalosporin and piperlonguminine as the research object, two aspects works has been done that the methods of determination was established and the interaction between drugs and protein was studied.In this work, take o-Nitrophenylfluorone (o-NPF) and2,3,7-Trihydroxy- 9-dibromo-hydroxyphenyi fluorone (DBH-BF) as electron acceptor with cefazolin morpholine (CFZ), PLG, and cefoperazone (CPZ) generate charge transfer complex respectively. Discussed the spectral properties of absorption spectrum of charge transfer complexes which are between o-NPF and CFZ and between o-NPF and PLG in detail, and discussed the spectral properties of absorption spectra, fluorescence spectra and resonance Rayleigh scattering spectra of the charge transfer complex which is between DBH-BF and CPZ, optimized the conditions of each charge transfer reaction, and explored the mechanism of charge transfer reaction. In charge transfer reaction system between o-NPF and CFZ, CFZ concentration within the range of4.545～172.72μg/mL follow Beer’s law, the apparent molar absorptivity of5.41×104L·mol-1·cm-1, the detection limit of1.53μg/mL, the recovery of the biological samples is within of99.8%-100.9%, this method has high sensitivity, fast, easy and accurate features, compared with the method reported. In charge transfer reaction system between o-NPF and PLG, PLG concentration within the range of4.545～72.72μg/mL follow Beer’s law, the apparent molar absorption coefficient of6.571×104L·mol-1-cm-1, the detection limit of0.0153μg/mL, the recovery of the biological samples is within of99.7%～100.0%, this is the first spectrophotometry to determine the concentration of PLG by charge transfer reaction, which has the advantages of accurate, simple, rapid, high sensitivity. In charge transfer reaction system between CPZ and DBH-BF, generated a charge transfer complex, the composition ratio of1:1, resulting in system absorption spectrum changes, fluorescence quenching and resonance Rayleigh scattering (RRS) intensity weakened, three methods of linear range of0.0668～0.6677μg/mL,0.03338～2.336μg/mL and0.03338-3.3383μg/mL, the detection limit of0.022μg/mL,0.0116μg/mL and0.0132μg/mL respectively, established three kinds of rapid, simple, accurate methods to determine concentration of CPZ in the injection and biological samples, and found that these methods are greatly improved in selectivity, sensitivity and measurement range by comparison with existing methods.In the interaction of drugs and protein, the fluorescence quenching reaction of CTX, PLG and BSA was studied by the absorbance spectra and fluorescence spectrometry, experiments showed that the process of two drugs with BSA was the static quenching process, the binding constant, the binding site number, the binding of the distance and the corresponding thermodynamic parameters are measured, the type of force between two drugs and BSA is identified, established the binding sites of CTX, PLG with BSA are mainly located in sites I of sub-domain Ⅱ by mark competition experiments, learned that negative synergy between the two drugs by calculate the Hill’s coefficients of the ternary system. Then the binding characteristics of CTX, Cu (Ⅱ)and BSA have been studied by fluorescence spectroscopy Cu (Ⅱ), the results showed that both Cu(Ⅱ) and CTX could quench the fluorescence intensity of BSA via a nonradiative energy transfer mechanism, CTX could quench the fluorescence of BSA significantly in the presence of Cu (Ⅱ), The binding constants and the binding sites were calculated respectively, accordingly,knowed that Cu (II) may be used as bridge to forming a ternary complexes which is CTX-Cu-BSA. Synchronous fluorescence spectroscopy and three-dimensional fluorescence spectrometry are used to investigate the structure change of BSA, when joined CTX to BSA.