| Vaccinium bracteatum Thunb. is a Chinese traditional medicinal plant, which is of Vaccinium(Erioaoeae). Polyphenol was found in Vaccinium bracteatum Thunb. fruits, leaves and flowers, which can anti-oxidation, anti-ageing, improve and prevent eye disease, anti-microbial, anti-cancer, and so on. In order to make better use of the wild resource of Vaccinium bracteatum Thunb., its fruits was used as material in this paper, the different extraction methods together with experiment design were studied to optimize the extraction technology of anthocyanin, and the anthocyanin extractive was divided into different polarities of solvents, then the activity of anti-oxidation and anti-microbial was studied. The main results as follows:(1)Base on the single-factor experiment, orthogonal experiment was used to optimize the extraction technology of anthocyanin from Vaccinium bracteatum Thunb. fruits, and the total anti-oxidation activity of anthocyanin on different condition was studied. The results showed that:the optimum parameters of extraction technology of anthocyanin were:ethanol concentration50%, ratio of liquid to material1:30g/mL, temperature50℃, time30min, times2. Under that technology conditions, the extraction rate of anthocyanin reached92.42%. And the total anti-oxidation activity of Vaccinium bracteatum Thunb. fruits was positive linear correlation with the contents of anthocyanin (p<0.01), that is, the anti-oxidation activity of Vaccinium bracteatum Thunb. fruits was mainly associated with its contents of anthocyanin.(2)Base on the single-factor experiment, quadric regression orthogonal rotary test was used to study the factors includes enzyme dosage, temperature and time, and so on, which influence the composite enzymatic extraction technology of anthocyanin from Vaccinium bracteatum Thunb. fruits, and mathematic formula was built. The results showed that:the optimum parameters of extraction technology of anchocyanin were:enzymes dosage234U/g, ratio of enzyme activity2:1, temperature50℃, time180min, pH4.0, ratio of liquid to material1:30g/mL, and through verification, the content of anthocyanin was137.61mg/100g, the rate of extraction was98.68%, which increased by18.8%compared to ethanol-extraction individual (times1). (3) The anti-oxidation activity of anthocyanin extractive and its different polarities of solvents from Vaccinium bracteatum Thunb. fruits was studied. The results showed that:the anti-oxidation activity of anthocyanin extractive was weaker than the polarities of solvents on the three anti-oxidation systems. The order of the activity of scavenging on-OH was:ethyl-acetate phase> ascorbic acid> n-butyl-alcohol phase> chloroform phase> anthocyanin extractive, the order of the activity of scavenging on02-·was:ethyl-acetate phase>ascorbic acid>chloroform phase≈n-butyl-alcohol phase>anthocyanin extractive, and the order of the activity of inhibiting lipid per-oxidation was:ethyl-acetate phase>chloroform phase-n-butyl-alcohol phase>anthocyanin extractive>ascorbic acid. Through qualitative and UV-Vis absorption spectral characteristics experiment, the ethyl-acetate extraction part was judged into anthocyanin.(4)The anti-microbial activity of ethyl-acetate phase was studied, and the factors including temperature, pH, time, and so on, which influence the inhibition effect of Saccharomyces cerevisiae was discussed. The results showed that:the ethyl-acetate phase had worse anti-activity for fungi, even advanced the growth of yeasts, such as Saccharomyces cerevisiae. But it had the stronger anti-activity for bacteria, the degree of anti-bacteria activity was:Escherichia coli> Bacillus subtilis> Staphylococcus aureus, and the minimal inhibitory concentration were0.625mg/mL,1.25mg/mL,1.25mg/mL.The several factors also had significantly effect on anti-bacteria, the anti-microbial activity of ethyl-acetate phase was stronger in normal or low temperature and in neutral or partial acidity; at the same time, sugar or salt can improve the anti-microbial activity. |
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