Study On Preparation And Application Of Luteolin Molecular Imprinted Polymeric Microspheres

Molecular imprinting technique is a new technology which the principle simulateto the interaction between antigen and antibody. Setting the target molecule whichneed to separate as template,through the polymerization reaction to get a kind ofpolymer which have specific selective to a particular matter. The preparation processeasy to control, and property is steady. Now, the technology is widely used.Natural products active ingredients is a main source which could prevent andcure the disease of human, and the active ingredient is basis of the function matter.Luteolin is a kind of flavonoid compound, There are a variety of pharmacologicalactivity, such as antitumor, antivirus, oxidation resistance, fight bacterium diminishinflammation, etc.Peanut shells is a kind of cheap and renewable abundant natural resource.Luteolin, a major flavonoid in peanut hull. Through the extraction and separation toget high purity of luteolin, which could be used for raw material for drugs or healthfoods, having a broad application prospect in the market.The contents of this paper mainly include as list:1. Luteolin was used as template molecule, under the action of functionalmonomer and crosslinking agent, add polyethylene glycol2000as dispersant, tolueneas porogenic agent, synthetized luteolin molecularly imprinted polymer microspheresin the condition of aqueous solution.The structure of molecular imprintingmicrospheres were characterized by infrared spectrometer,laser particleanalyzer,Liquid nitrogen adsorption instrument,ect. Examined the preparationconditions’effect to the adsorption quantity and polymer morphology, gaining thebetter preparation conditions.2. The static adsorptive method was used to analyse the adsorption performanceof luteolin in the molecularly imprinted polymer microsphere.The isothermaladsorption indicated that the biggest adsorption quantity was different betweenimprinted and not imprinted molecularly imprinted polymer microspheres.Accordingto scatchard equation analysis could be informed that: in certain concentration range,molecularly imprinted polymer microsphere formed two different binding sites.Through calculating the balance equation of dissociation constants, the results were0.2198mmol/L and2.7603mmol/L. The isothermal adsorption indicated that thebiggest adsorption quantity were53.6mol/g and223.4mol/g. The adsorption isotherm could be correlated by Langmuir isotherm equation. Properly raisingtemperature was benefit to adsorption. The adsorption dynamics could be wellcharacterized by Lagergren level2rate equation. The absorption rate constantincreased with the rising of temperature.3. Molecularly imprinted polymer microsphere and blank molecularly imprintedpolymer microspheres were treated as the stationary phase of chromatographiccolumn. The luteolin and its analogues were isolated by the chromatographiccolumn.The results showed that the molecularly imprinted polymer microspheres hadgood selectivity to luteolin.4. Molecularly imprinted polymer microsphere was treated as the stationaryphase of chromatography column. Chromatography column would be directly usedfor extracting luteolin from peanut shells. Through the optimization ofchromatography column separation conditions, choosing70%ethanol solution toelute extractive at the speed of0.5mL/min，after that，the purity of extractives ofpeanut shells of luteolin was increased from2.7%to90.0%, and recovery rate was92.7%。...