Microflow Injection Analysis Determination And Application Based On NaClO-Luminol-PhloxineB Chemiluminescence Resonance Eenergy Transfer System

Chemiluminescence (CL) detection combined with flow injection analysis (FIA) or sequential injection analysis (SIA) has advantages of high sensitivity, high analytical throughput rates with relatively simple instrumentation, versatility and real-time continuous monitoring. Miniaturization of chemical assay on microfluidic system is generally recognized as one of the most important trends in the development of analytical instrumentations. The microflow injection analysis system has been developed for its low sample and reagent consumption, reduced analysis time and suitability for miniaturized systems. However, since the channels in micro-devices are extremely small, the number of molecules actually available for detection decreases with the decrease of reagent and sample consumption. It is of great significance to enhance the sensitivity of chemiluminescence detection, especially for the miniaturized analytical instrumentations.In the first chapter, flow injection-chemiluminescence system, chemiluminescence resonance energy transfer (CRET), the application of flow injection chemiluminescence system for the detection of hypochlorite and the detection method of phloxine B were reviewed.In the second chapter, we reported for the first time that the sensitivity of luminol-hypochlorite chemiluminescence reaction was enhanced significantly by the addition of phloxine B and discussed the mechanism of the reaction-chemiluminescence resonance energy transfer. The effects of pH of media, flow rate of CL reagent, correlated condition of the mixture of CL reagents and sensitizer on the detective sensitivity were also investigated. Experimental results proved that the proposed method could be used for the determination of trace hypochlorite in water. It is anticipated that an on-line hypochlorite/chlorine analyzer would be developed in the near future for real-time continuous monitoring hypochlorite/chlorine in tap-water and other waters with high sample throughput (90h-1), less reagents consumption (2.0μL), low operating cost and low maintenance.