The Chemiluminescence Resonance Energy Transfer Research On Monitoring Of Formation Dynamics Of Singlet Oxygen

Singlet oxygen?¹O₂?is the ultimate cytotoxic agent required for effective photodynamic therapy?PDT?.So far,several strategies have been attempted to monitor the production of ¹O₂,but the sensitivity is low and this lacks enough information involved in the formation dynamics of ¹O₂.Therefore,the development of innovative approaches for rapid evaluation of photosensitizers by virtue of the real-time monitoring of ¹O₂ is one of the biggest challenges.The aggregation-induced emission?AIE?-active fluorophores,which can induce emission highly in the aggregated state or solid state could improve the chemiluminesence?CL?resonance energy transfer?CRET?efficiency between ¹O₂ donors and fluorescent molecules.However,the aggregation-induced emission surfactant has the disadvantages of low solubility and poor biocompatibility,and it is necessary to further develop a novel targeted fluorescent probe to realize the screening of photosensitizers in vivo.In this study,two systems were established based on ¹O₂ in vivo and in vitro monitoring.1)Study the application of TPE-SDS based on the principle of CRET in the detection of ¹O₂.TPE-SDS could form a cage-like structure during the self-assembly process,improving the CRET efficiency between ¹O₂ donor and TPE-SDS acceptor by shortening the distance between ¹O₂ and TPE luminophor.The fabricated CL platform good analytical performances for the detection of ¹O₂ with a wide linear range?1.0-100 ?M?,good reproducibility and stability and had been used for screening three photosensitizers?rose bengal,rhodamine 101,and riboflavin?by monitoring the formation dynamics of¹O₂ usin exhibited g a light-emitting diode light source.2)A fluorescent liposome with good biocompatibility and stability was synthesized by hydrothermal method.The size of liposomes was about 5-10?m.In the process of forming liposomes,C₄₆H₈₄NO₈P is self-assembled from the free state to form an ordered bimolecular layer structure,the ester groups in C₄₆H₈₄NO₈P form enol,and the orderly combination of C₄₆H₈₄NO₈P molecules with hydrogen bonding linkage were assigned as the emitting sources.The effects of fluorescent liposomes on the CL intensity of ¹O₂ and the mechanism were investigated.Due to the good biocompatibility of the liposomes,it is expected to achieve the detection of ¹O₂ in vivo.