Study On Submerged Fermentation With Paecilomyces Hepialid

Cordyceps sinsnesis, an expensive traditional Chinese medicine, has attracted a greatdeal of attention due to its multiple pharmacological functions,such as replenishing kidney,smoothing lung, and anti-tumor activity. However, its usage has been limited due to the longgrowth period and over-exploitation of natural cordyceps sinsnesis. Resently, the submergedculture of Paecilomyces hepialid （derived from the genus Cordyceps） have been developed. Itis generally accepted that the medicinal merits of cultured mycelia are similarly effective asthose of Cordyceps species found in the wild.However, the cordyceps mycelia obtained by thesubmerged culture was limited due to low pharmacological composition content and excessplumbum and arsenic. In this study. the influence of plumbum and arsenic on Paecilomyceshepialid fementation was firstly investigated.Then, the culture condition was optimized bysingle factor experiment and orthogonal design for its industrial application.The results showed that the plumbum had little effect on the paecilomyces hepialidfermentation, but the arsenic showed high cell toxicity on the mycelia. The curves describedby the lead and arsenic concentration in the mycelia and the culture medium fitted well tolinear equations. The composition of optimized medium for polysaccharide production was3.0%glucose,4.0%sucose,2.5%soybean powder,1.0%peptone,0.1%KH₂PO₄, MgSO₄0.05%,with a maximum polysaccharide concerntration of5.115%.When the pH wascontrolled at pH4.0, the polysaccharide concentration reached peak of9.3%in a5Lbatch-fermentor.