A Preliminary Study On The Wastewater Treatment Process In Urease And Phosphatase Purification Of Wetland Plants And Rhizosphere Microorganisms

Artificial wetlands have various advantages, the importance of them has becomeincreasingly prominent. How to make better use of it to solve environmental problems,has been the focus of researchers. Decontamination effect of wetland plants,purification mechanism and preparation basis have been studied. Some of thesubstances secreted by the roots of plants, such as enzymes, sugars, organic acids, andgrowth hormone, etc. may be used as the microbial nutrients to promote their growth.The microorganisms of research include microbial nitrogen cycle in general,methanogenic archaea and methane oxidizing bacteria, sulfate-reducing bacteria andsulfur-oxidizing bacteria. Studies of the combined effects of the plant and microbialplants are almost in the pattern of one or more efficient degrading bacteria with plants,especially immobilized nitrogen cycling bacteria and heterotrophic denitrificationbacteria.About phosphatase and urease, soil enzyme activities can indicate biologicalactivity in the soil, including soil urease and the peroxidase are closely related to totalnitrogen.alkaline phosphatase activity may reflect the microbiological condition inthe soil, and their activity have a direct relationship with nitrogen, phosphorus andpotassium in the soil.In our Laboratory plant catalase and superoxide dismutase were studied. In orderto better determine the role of the enzyme, we studied the change of urease andphosphatase in Plants and secreting into the environment in various environmentalconditions，include of microorganisms on the contribution of these enzymes in theenvironment. Through the monitoring of urease and phosphatase to know enzyme inthe role of pollution control and remediation of water. The conclusions as follow:（1） R.japonicus Thunb., Typha orientalis，S.trisetosus Tang et Wang，Rumexcrispus Linn.，Scindapsus aureun all have a certain self with sewage treatment effect.root acid phosphatase, alkaline phosphatase and urease activity in the five plants havethe same trend within sewage treatment group and the control group, but do notcoincide. basically blank control group have the higher level of activity in sewagetreatment groups.（2）The five plants’ root alkaline phosphatase and sewage TP and COD₅therewas a strong correlation, but there was a significant correlation between the part of theplants’ roots in alkaline phosphatase and TP and COD₅.So alkaline phosphatase playsa certain role in phosphorus removal,but may not be the only way to phosphorusremoval,and it can be used as reference indicators when screening of wetland plants.（3）Individual plant treatment group and between microorganisms and plantscombined treatment group, the latter of sewage ammonia, COD₅lower, to reflect the synergies between microorganisms and plants. Urease activity of urease activity inwater three wetland plant trend in water of different microorganisms and plantscombined treatment group is compared with little change. Separate plant treatmentgroup urease in the water activity level to be higher than the correspondingcombination group of microorganisms and plants. Acid alkaline phosphatase in thewater the vitality level trend consistent in each treatment group were increased withtime. Ultimately the same kind of plant microorganisms and plants combinedtreatment group and acid phosphatase activity level of the individual plant treatmentgroup to achieve the same, but the latter activity increased faster than the former.（4） Microorganisms and plants combined treatment group and individual planttreatment group’ water urease and ammonia nitrogen in water, although not verysignificantly related, but were obvious close to reach a significant level.Microorganisms and plants in the combined treatment group was significantly greaterrelationship than the individual plant treatment group, while only plus bacteria ureasein water and ammonia in the control group was highly significantly related. Whenmicroorganisms exist, the correlation between urease in the water and ammonia levelis higher.