Synthesis, Characterization And The Interaction With BSA And DNA Of Amide-based Open-chain Crown Ethers And Their Rare Earth Complexes

The anticancer target molecules-protein and nucleic acid have vitally important physiological functions. So the interaction mechanisms on these biomacromolecules with drug molecules is one of current interest in clinical medicine chemistry and biology. Rare-earth elements and their complexes have significant anti-infiammatory, antibacterium,anticoagulation,antineoplastic and other drug activity. Compounding with a ligand which possess a certain drug activity,rare earth complexes will not only be more active,their toxicities also be weakened. In this paper, rare earth complexes were synthesized with picorate rare earth and amide-base open-chain crown ligands which also have drug activity. The interactions of target complexes with BSA and were studied.The main work of the thesis is:(1) There amide-base open-chain crown ligands(L1～L3) were synthesized with2,7-Dihydroxynaphthalene and different end-group. Its rare earth picorate complexes[RE(pic)3]L2(RE=La、Ce、Eu、Tb) were characterized by’HNMR, IR spectra, elemental analysis, molar conductivity and TG-DSC.(2) The interaction of picorate Ce(Ⅲ), ligands(L1～L3) and picorate Ce(III) complexes with BSA was studied by fluorescence spectrum in Tris-Hcl buffer solution(pH=7.30) and they all caused the fluorescence quenching BSA by a static quenching procedure. The binding constants (KA) was Ce(pic)3> complexes> ligand and the number of binding sites was Ce(pic)3> complexes(ligand). The results indicated that the target complexes really have synergistic effect or unique performance of Ce(pic)3and amide-base open-chain crown ligand.(3) A series of the complexes with DNA is intercalation which was studied by UV spectra, fluorescent spectra, cyclic voltammetry and viscosity. After the DNA was added, the strongest UV absorption bands of complexes showed hypochromism and blue shift. The fluorescent intensity of the EB-DNA system weakened with addition of complexes. Adding DNA into the complexes solution results in the decrease of the oxidation peak currents declined and the peak potential positively shift.The relative viscosity of DNA was increased with addition of complexes.