Sulfa Drugs In Marine Fish Metabolism Kinetics Study

"Food security is the first", with the increasing people’s material life, food type and quantity of great wealth, the people meet the basic needs of life under the premise of food safety concerns gradually improved, goverments around the world are mostly food safety as a national public safety, increasing supervision. However, due to the structure, mechanism and other aspects of the problem and in recent years, China’s food safety incidents, but despite repeated prohibitions. Protect people’s health, and enhance the international competitiveness of agricultural products in China, we must promote the detection of food-borne hazards key technologies and agricultural safety control technology innovation and development. To establish a sensitive and accurate drug residue detection methods is to protect the quality and safety of agricultural products of an effective defense, understand and master the metabolism of the drug in vivo kinetics medication guide is to protect the fundamental quality and safety of agricultural products.Strengthen supervision of drug residues in aquatic products, you first need to solve these two issues is what.In this paper, to find effective method for extraction of sulfonamides by chemical properties, according to the different tissue properties, extraction liquid purification method, and then optimize the liquid chromatography mass spectrometry instrument conditions so that a variety of sulfa drugs to achieve effective separation, making detection of multiple kinds of sulfonamides residues in aquatic products, the method the detection limit was0.01μg/kg～0.2μg/kg, the limit of quantification was0.07μg/kg～0.67μg/kg, the average recovery rate was68%～82%, the relative standard deviation is4.1%～11.8%, sensitivity than traditional method increases more than ten times, not only solves the problem of not fully separated by high performance liquid chromatography, and methods of qualitative and quantitative and reproducible, accurate, high sensitivity, suitable for multiple parameters, large quantities of samples detect.This paper studies on specific temperature, different kinds of sulfonamides in metabolic kinetics of different kinds of sea water fish, metabolic kinetic parameters of different tissues are obtained and withdrawal period parameters.At the temperature of (14±1)℃, with100mg/kg (by body weight) single dose of Sebastodes fuscescens perfusion of sulfamethazine, liver, blood, muscle Tmax were:6h,8h,10h, Cmax respectively, the peak concentration of26.45μg/g,25.57μg/g and31.35μg/g. The liver, blood and muscle elimination half-life of4.78h,19.97h and22.22h, respectively. SM2in the blood at lOd below the residue limit is0.1mg/kg, the drug residues in muscle tissue concentrations of14d was lower than0.1mg/kg, and the liver drug concentrations below the MRL requires22d. At the temperature of (11±1)℃, in accordance with the dose of100mg/kg (by body weight) after oral administration, plasma after administration of0.5-4hours, plasma drug concentration rose rapidly, and in4.87hours after administration of the maximum of44.07μg/mL, drug concentration began to drop,10days later not detected (<10μg/L). Muscle after administration of0.5to6hours, the rapid growth of the drug concentration in muscle,6hours after the growth slowed down, in11.89hours after ad ministration of the maximum of21.52μg/g, drug concentration began to decline,5days later, the drug concentration of0.097μg/g, below the maximum residue limit (0.1μg/g)18days later, not detected (<10μg/kg). Liver after administration of10.53h in the liver concentration reaches a maximum value (Cmax=5.35g/g), but the value is obviously lower than the drug concentration in muscle, rising trend is relatively gentle.72h after administration, drug concentration to0.91μg/g, drug metabolism is very slow, drug concentrations below the maximum residue limit (0.1μg/g) takes6days,24days not detected (<10μg/kg).To sum up, drugs in fish body absorption, distribution, metabolism and elimination, except with differences between different organizational functions, water temperature, fish species, fish drugs are factors that can affect, in choosing a drug that should not only take into account the above factors, also according to the period to determine the reasonable use of time and dose, ensure efficacy and safety should be fully considered.