Isolation Of Electrogenic Bacteria And Research On Electrical Performance Of MFC Form Organic Waste

Because microbial fuel cell both has the role of governance pollutants andbiomass power generation, it has increasingly become the focus of scholars. Atpresent, the use of organic waste as a substrate for microbial fuel cells at home andabroad are less. Organic material, as a solid waste, have the features of riching inorganic matter content and having a huge number. If we can use microbial fuel cellsto resource utilization organic waste, we will have considerable benefits and reducethe burden of organic waste disposal.The fold plating methods were adopted for isolation from biofilm incompartments of an “H” typed microbial fuel cell. The strain, named Nitratireductorsp. WJ5-4, owned the highest electrogenic activity in compartment. First,investigated the individuals, colonies, Gram stain,16SrDNA identification of thebacteria. The result is the strain belongs to Nitratireductor genus, gram-negativebacilli, small colonies, round, smooth edges, milky white, named Nitratireductor sp.WJ5-4.Investigate the physiological and biochemical characteristics ofNitratireductor sp. WJ5-4to optimize the cultivation and production of electricaltest conditions. There are Growth factor, temperature, anaerobic and carbonutilization. Results of the analysis showed that Nitratireductor sp. WJ5-4growthrate and final concentration were significantly increased when0.1g/L of yeastextract was added into the culture medium; the optimum pH value is6.5;growth under35℃was slightly better; could use a range of carbon sources,including glucose, urea, lactose, ammonium citrate.Different concentrations of glucose and different carbon sources were chosenas carbon source in anode chamber. When inoculated with100ml broth and theconcentration of glucose was11g/L, Nitratireductor sp. WJ5-4has a voltage of290mV with the maximum power density96.13mW/m~2, the minimum internalresistance635.22and higher COD treatment efficiency77.03%. When using ureaas carbon source, system has a voltage of450mV with the maximum power density101.4mW/m~2and higher COD treatment efficiency77.47%.Organic waste (crushed rice) was chosen as carbon source in anode chamber.Design a blank experiment, the substrate and the device is the main reason of the initial voltage of the MFC. When inoculated with200ml broth and keep thesubstrate500g, system has a voltage of334mV with the maximum power density128.07mW/m~2and higher TOC treatment efficiency41.46%. When keepinoculated with200ml broth and the substrate400g, system has a voltage of434mV with the maximum power density163.69mW/m~2and higher TOC treatmentefficiency46.29%. So, keep inoculated with200ml broth and keep the substrate400g, system can achieve the capacity threshold.