Preparation And Application Of Molecularly Imprinted Polymer Using Plasticizer And Olivetol As Templates

Molecularly imprinted polymer (MIP) possesses excellent selectivity and chemical stability, which isappropriate to be used as the absorbtion materials of solid phase extraction (SPE) and solid phasemicroextraction (SPME) for separation and enrichment of complex samples. Recent years, molecularlyimprinted solid phase extraction (MI-SPE) and molecularly imprinted solid phase microextraction(MI-SPME) have been widely used to analyze environmental samples, biological samples and foodsamples. This paper mainly studied the application of SPE and SPME technology in food analysis. Thethesis consists of six chapters:In chapter1, we introduced the principle of molecular imprinting, preparation method and applicationof MIP, mainly focused on the application of MI-SPE and MI-SPME in food analysis.The preparation and application of dioctyl phthalate (DOP) MI-SPE cartridge was introduced inchapter2. MIP was synthetized by bulk polymerization, using DOP as template molecular. Staticadsorption and selective experiments suggested that MIP had a good selectivity for DOP. Then MIP wasused as the absorbtion materials of SPE, and a method for determination of the three kinds of PAE inbeverage by the MI-SPE-HPLC was developed. The linear range of this method was0.1-10mg/L, therecoveries for the three PAE were up to90.4-97.8%, the relative standard deviations (RSDs) and the limitsof detection (LODs) were found in the range of1.6to3.8%and0.008-0.018mg/L, respectively.The preparation and application of dimethyl phthalate (DMP) MI-SPME fiber was discussed inchapter3. With the help of microwave irradiation, monolithic MIP fiber was prepared by in situpolymerization in the capillary. Selective experiment showed that MIP fiber had a good selectivity for DMP.A method for determination of the three PAE in beverage by MI-SPME-HPLC was developed. Theproposed method was linearity in the range of0.5-100μg/L, the relative recoveries of the three PAE wereranging from73.8to98.5%, the relative standard deviations (RSDs) and the limits of detection (LODs)were within3.6-6.8%and0.08-0.2μg/L, respectively.The preparation and application of olivetol MI-SPE cartridge was studyed in chapter4. MIP wassynthetized by bulk polymerization, using olivetol as template molecular. Static adsorption and selectiveexperiments suggested that MIP had a good selectivity for olivetol. Then MIP was used as the absorbtionmaterials of SPE, and a method for analysis the olivetol in the wheat bran by MI-SPE-HPLC was developed. The linear range of the established method was0.1-100mg/L, the recoveries for the olivetolwere ranging of97.8-98.8%, the relative standard deviations (RSDs) and the limit of detection (LOD) werefound within2.8-4.2%and0.062mg/L, respectively.The preparation and application of olivetol MI-SPME fiber was investigated in chapter5. With thehelp of microwave irradiation, monolithic MIP fiber was prepared by in situ polymerization in the capillary.Selective experiment showed that MIP fiber had a good selectivity for olivetol. A method for analysis theolivetol in the wheat bran by MI-SPME-HPLC was developed. The presented methord was linearity in therange of0.5-10mg/L, the relative recoveries of the three PAE were up to90.5-93.6%, the relative standarddeviations (RSDs) and the limits of detection (LOD) were4.9-6.0%and0.026mg/L.Chapter6was a summary and outlook of full paper. A detail summary about our research was made,and the suggestions and opinions on the problems existing in the paper were put forward.