| As a highly efficient biocatalyst, the enzyme in the nature can take part inthe vast majority of reactions. In addition, the analysis conducted tests that useof enzyme has wide application in many fields. However, inconvenience tostorage as well as their instability and other reasons, limit the application ofenzyme in some field. Therefore, researchers have paid more and more attentionto the synthesis of mimetic enzyme recent years, aims to that the mimeticenzyme is similar in properties and functions with natural enzyme. So far,scientists have found that metal oxide nanoparticles such as Fe3O4and Co3O4,graphene oxide and double inert metal composite nanoparticles such as Au@Ptand many other inorganic nanomaterials all have the properties of peroxidase, aswell as these substance has a lot of values in analytical detection and pollutiontreatment.In this thesis, we prepare cupric oxide nanoparticles by the method ofprecipitation and prepared Cu-SBA-15by the hydrothermal process. In order todetermine the morphology and size of cupric oxide and the pore structure of Cu-SBA-15， and the cupric in the framework, we characterize with X-raydiffraction, scanning electron microscopy, nitrogen adsorption-desorption andinfrared spectrometry, and so on.We demonstrate the catalytic activity of mimetic peroxidase enzyme incupric oxide nanoparticles by a series of enzyme activity test. The material cancatalysis hydrogen peroxide to oxidize3,3’,5,5’-Tetramethylbenzidine, and weget to know that the best pH value is5.0, best temperature is40℃and bestsubstrate concentration including hydrogen peroxide and3,3’,5,5’-Tetramethylbenzidine in the reaction of cupric oxide nanoparticles as themimetic enzyme,as well as the material’s stability of the properties in pH andthermal, is better than horse radish peroxidase. Besides these, we find theprocess of cupric oxide nanopaticles catalytic reaction is fit to Michaelis-Mentenequation, and calculate the Michaelis constant of hydrogen peroxide and3,3’,5,5’-Tetramethylbenzidine.We build a optical analysis method to detect the concentration of hydrogenperoxide and glucose by cupric oxide nanopaticles as the mimetic peroxidaseenzyme. Linear range of detecting hydrogen peroxide is from1.0×10-5mol·L-1to2.0×10-4mol·L-1, limit of detection is6.0×10-7mol·L-1. Linear range ofdetecting glucose is from6.0×10-5mol·L-1to1.0×10-3mol·L-1, limit of detectionis4.0×10-6mol·L-1. We take advantage of the detecting method to detect concentration of blood glucose, to our joy, the method has high accuracy rating,the detecting values are very similar to the value detected by hospital. Therecovery is from98.97%to101.26%, and the relative standard deviation isbetween2.39%and4.08%.We demonstrate the catalytic activity of mimetic peroxidase enzyme in Cu-SBA-15by a series of enzyme activity test. The material can catalysis hydrogenperoxide to oxidize3,3’,5,5’-Tetramethylbenzidine, and we get to know that thebest pH value is4.0and best temperature is50℃in the reaction of Cu-SBA-15as the mimetic enzyme,as well as the material’s stability of the properties in pHand thermal, is better than horse radish peroxidase.We build a optical analysis method to detect the concentration of hydrogenperoxide and glucose by Cu-SBA-15as the mimetic peroxidase enzyme. Linearrange of detecting hydrogen peroxide is from8.0×10-4mol·L-1to6.0×10-2mol·L-1, limit of detection is3.7×10-6mol·L-1. Linear range of detectingglucose is from4.0×10-3mol·L-1to8.0×10-2mol·L-1, limit of detection is5.4×10-6mol·L-1. We take advantage of the detecting method to detectconcentration of blood glucose, to our joy, the method has high accuracy rating,the detecting values are very similar to the value detected by hospital. Therecovery is from99.59%to101.42%, and the relative standard deviation isbetween0.53%and2.63%. |
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