Research Of The Stengthen Transformation Capacity Of The Potato Protein Microbial

Potato waste is the byproduct of potato starch industry. When the byproduct isreleased into the environment, it not only causes a great waste, but also generats greatlyload to the Environment, which is contrary to the innovative idea of “turning waste intotreasure, cleaner production”. Our experimental center make use of the potato waste asfermentation medium,access microflora for its resource processing production of singlecell protein (SCP). In this study, Saccharomyces cerevisiae and Cyberlindnera jadinii areused as the research object, in order to improve the quality of potato protein microbialand their conversion capabilities，the medium is optimized by the way of adding glucose,and deal the strains with UV mutagenesis，then use the response surface optimizationmethod for further Optimization of the fermentation conditions.First, each component of the potato residue is measured in order to find the reasonfor the poor ability of producing SCP. Maybe because there is too little sugar in themedium can be used.So we add glucose to the fermentation medium to optimize theproduction of SCP, and find how much glucose add to the potato waste medium theproduction of protein of the two kinds of yeast can reach the highest, studies have shownthat when add28mg/mL glucose, Saccharomyces cerevisiae get the highest yield,7.683mg/mL, whose protein conversion rate is42.49%and add42mg/mL glucoseCyberlindnera jadinii get the highest yield6.994mg/mL，whose protein conversion rateis38.41%. On this basis, deal the two yeast with UV mutagenesis and then we havescreened three production significantly enhance and genetically stable strains: S57,whose protein yield is8.425mg/mL with the protein conversion rate46.88%, U112,whose protein yield is8.429mg/mL, with the protein conversion rate46.90%.Finally, mixed culture the high-yielding strains of mutagenesis and explore researchits best fermentation condition by the way of response surface optimization. Through theexperimental analysis,the best fermentation conditions is27.7°C, pH7.70,187.11rpm,mixed bacteria ratio1:1, the mass fraction of35mg/mL glucose added, inoculum1%, thetheoretical maximum protein yield is9.016mg/mL. The protein yield is9.185±0.048mg/mL in the proven test, and its protein conversion rate is51.38%. The determinationof COD removal efficiency is66.7%, which compared to the previous60%has notreceived a greatly improved. because the the cellulose in the juice has not been processedin this experiment.