The Synthesis Of Ⅱ-Ⅵ Group Semiconductor Nano-Fluorescent Probes And Their Application In Pharmaceutical Analysis

The new type of fluorescent nanoprobe with its special quantum confinementeffects and small dimension effects exhibits many different optical characteristicscompared to the homogeneous single-molecule or large object, and thus has attractedgreat attention in the applied research field on analytical chemistry. Among thefluorescent nanoprobes, semiconductor nanoparticles have been developed into newnano-fluorescent probes due to possess many advantages, such as narrower emissionspectra, tunable maximum emission wavelength with changeable sizes andcompositions, photostability, high brightness, long fluorescence lifetime andbiocompatibility. Recently, semiconductor nanoparticles have become a new hotspotand they are widely used in fields like analytical chemistry, biological medicine andlife sciences.In this paper, the main research will be in the synthesis of II-VI groupsemiconductor nano-fluorescent probes and their application in pharmaceuticalanalysis. The phenomena of fluorescence quenching and enhancement ofnano-fluorescent probes in the presence of some medicines with weak or withoutfluorescence were researched, and thus sensitive methods for specific drug analysiswere proposed. The major results are as follows:1Hydrothermal synthesis of functionalized ZnS nanoparticles and their applicationas fluorescence probes in the determination of nevirapine. First, ZnSnanoparticles with defined shape were synthesized by hydrothermal synthesismethod, and the average particle diameter was50nm. And then the thioglycollicacid was used as capping agent. After that the functionalized ZnS nanoparticlesworked as fluorescent probes for the quantitative determination of nevirapine.Under the optimum conditions, the relative fluorescence intensity （log（F₀/F）） ofZnS nanoparticles is linearly proportional to the nevirapine concentration ranging from4.6to200μM with the correlation coefficient （r） of0.9996, and the limit ofdetection for nevirapine is found to be0.73μM. Moreover, the possiblequenching mechanism was also investigated was concluded to be the combine ofstatic and dynamic quenching. Finally, the present method was applied todetermine the standardized recovery of nevirapine in human serum, and theresults were satisfactory.2Facile aqueous synthesis of bovine serum albumin capped ZnS nanoparticles andtheir application as fluorescence probes for determination of gatifloxacin. First,the functionalized ZnS nanoparticles were successfully synthesized in aqueousmedium, with the biomolecule bovine serum albumin as the capping ligand. Theobtained products had uniform particle size and were about30nm in diameter.And then ZnS nano-fluorescent probes were employed in the detection ofgatifloxacin. Under the optimal conditions, a good linear relationship （r>0.998）between the log（F₀/F） and the gatifloxacin concentration in the range of0.2-100μM was obtained, with a detection limit of0.065μM. The mechanism studyshowed that bovine serum albumin capped ZnS nano-fluorescence probes werequenched by gatifloxacin through both dynamic and static quenching mechanisms.At last, the present method was applied to quantify gatifloxacin in gatifloxacincapsule and human serum by standard addition method, and the results weresatisfactory.3Synthesis of functionalized core-shell CdTe/ZnS nanoparticles and theirapplication as fluorescence probe for norfloxacin determination. The core–shellCdTe/ZnS fluorescence probes were prepared using inorganic salts as precursorsand thioglycollic acid as the stabilizer. Under the optimum conditions, thefluorescence intensity quenched gradually with the increase of norfloxacinconcentrations. In the concentration from0.2μM to140μM, the relativefluorescence intensity of the system is linearly proportional to norfloxacinconcentration, and obey to the following equation:F₀/F=0.001[Q]＋1.066（Q,0.1μM） with a detection limit of0.082μM. Moreover, the proposed method wasapplied to detect norfloxacin in norfloxacin capsule with acceptable results.