| The discovery of new herbicide targets and their lead structure is important for the design, synthesis and development of new herbicides. It is important to establish a simple, rapid and visualization method that can be used to confirm the localization of a target, which is import to promote the discovery of herbicide target. In this paper, the CdTe quantum dots fluorescent probe (CdTe-QDs-IAA) connected a-alkyl indole acetic acid was designed and synthesized, and co-incubated with the section of green beans radicle. By this way, we determined the distribution of the fluorescent probes in the section of green beans radicle, and establish a method for the localization of target proteins of herbicides. The main research works and the main achievements of this thesis are as follows:(1)Introduction of summarize the relevant background knowledges this paper, including the transport mechanism of auxin, the receptor protein of auxin, the target proteins of drug and the synthesis of quantum dots and its application in the biomedical fields.(2)The green, yellow and red-emitting CdTe quantum dots (CdTe-QDs) was obtained by aqueous synthesis method, using mercaptopropionic acid (MPA), glutathione (GSH) as the stabilizer. The structural properties and optical properties of the CdTe-QDs were characterized. The results showed that the synthesized CdTe-QDs have stable optical performance, with good distribution and uniform particle size (about 2～3.5 nm) and high fluorescence quantum yield (about 55～74%)(3)The red-emitting CdTe-QD-IAA fluorescent probes with different coupling rates were synthesized through covalent attachment between α-alkyl auxin indole acetic acid and CdTe-MPA-QDs with 1-(3-dimethylaminopropyl)-3-ethyl carbodiimide (EDC) as a coupling agent. Calculated the coupling rates of α-alkyl indole acetic acid on the surface of three different CdTe-QDs-IAA fluorescent probes by difference method. The synthesis of CdTe-QDs-IAA fluorescent probes were proved by dynamic light scattering (DLS), fourier transform infrared spectroscopy (FTIR), ultraviolet absorption spectroscopy (UV-Vis) and fluorescence emission spectra. The results showed that with the increase of coupling rate of α-alkyl indole acetic acid on the surface of CdTe-QDs-IAA fluorescent probes, the increase of the hydration particle size, the slight blue shift of the fluorescence peaks, the decreased of the fluorescence intensity, and the unchanged of the UV absorption spectrum.The fluorescence stability of the fluorescent probes were tested in different environments, indicating the stable fluorescent properties of them under physiological conditions.(4)The results of the toxicity tests showed that CdTe-QDs-IAA fluorescent probes were minimal toxicity to mung bean seedlings at low concentrations. The a-alkyl indole acetic acid binding site in mung bean radicle were marked by CdTe-QDs-IAA fluorescent probes incubated with the section of green beans radicle. The results showed that red-emitting CdTe-QDs-IAA fluorescence probes were capable of good resistance to plant tissue autofluorescence interference, and fluorescent probes mainly aggregation in cortical cell of mung bean root. Contrasted the biological activity of CdTe-QDs-IAA fluorescent probes and a-alkyl indole acetic acid using competitive adsorption method, the results showed that CdTe-QDs-IAA fluorescent probes synthesed have the biological activity of a-alkyl indole acetic acid. CdTe-QDs-IAA fluorescent probes were utilized to create a method for in situ detection of the fluorescent probe to establish a new method for auxin inhibitor target proteins distribution and localization in plant tissue, this method owns the advantages of simple and intuitive visualization.(5)The click reaction have important significance in quantum dots fluorescent probes biological target imaging studies because the specific functional groups of click reaction are small, biologically inert and absence in vivo. In this paper, the CdTe-QDs-N3 was synthesized, and The structural properties and optical properties were characterized. The results showed that the synthesized CdTe-QDs-N3 have stable optical performance and fluorescence performance in a short time. This laid the foundation for the subsequent click reaction in quantum dots fluorescent probes biological target imaging studies. |
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