| The magnetic separation enzyme-linked immunoassay (MEIA) is the technologyusing the superparamagnetic microspheres as separation media and it has the advantagesof high sensitivity and linear.Combining the flow injection analysis(FIA) technology,computer software and MEIA technology can achieve automatic magnetic separation andonline detection function; It also can improve the detection sensitivity, reduce theexperimental error and simplify the experimental operation through the automatic precisecontrol and continuous monitoring.Because of the obvious absorption and scattering of light, the detection limit andprecision of MEIA based on optical detection methods are affected by magneticmicrospheres. To detect antigen-antibody reaction with the direct magnetic properties ofmagnetic particles can eliminate these negative factors and to improve the detectionsensitivity and accuracy. In addition, the method combines magnetic signal detection withoptical detection can improve the accuracy and reliability of automatic analysis.In this paper, the hardware experimental platform of FIA combined with magneticseparation was constructed for MEIA. The LabVIEW virtual instrument measurement andcontrol software was written to realize the magnetic separation and on-line photometricdetection automatically. The chloramphenicol analysis based on optical-detection MEIAwas carried out on the FIA system; A magnetic detection device based on magneticinduction coil was constructed to study the superparamagnetic nanoparticles quantitativedetection technology for online MEIAThis paper mainly consists of the following work:(1)Construction of the magnetic separation enzyme-linked immunoassay systemshardware platforms based on the FIA:①Construction of the hardware control andflow control system based on the peristaltic pump, injection pump and solenoid valves.②Building a online monitoring system based on optical detection and magnetic separation.③finishing the test of the stability of the system.(2)Writing of the virtual instrument software of the hardware system:①Controlling the solenoid valves, peristaltic pump, and magnetic separation devices with the LabVIEWsoftware to realize automatic injection, nesting, and magnetic separation of this hardwaresystems.②Controlling USB4000for online photometric detection, access to test data,processing and displaying data.③The system test with realize the magnetic separationand on-line photometric detection.(3)A feasible study of the superparamagnetic nanoparticles for direct detectiontechniques: Building a magnetic detection device based on the magnetic induction coil;researching the influence of different measurement conditions and test methods on thequantitative measurement of the magnetic particles to realize the milligram scale magneticparticle detection. |
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