Study On The Collaborative Of Acaudina Molopadioides Autolysis And Exogenous Enzyme To Prepare Active Peptides

Sea cucumber is the animal belonging to Echinodermata Holothuriodea in general.In daily life, the word of sea cucumber is on behalf of the stichopodidae of seacucumbers. This kind of sea cucumber is in the highest nutritional value and succulentruannuo, more and more people like them. Acaudina Molopadioides is the one of thesea cucumber of Molpadida. Because of thick fleshy and difficult to cook, they havebeen neglect by people. Now people have found Acaudina Molopadioides have a highnutritional value. At the same time, people also have found Acaudina Molopadioidesthat is stimulated by certain external conditions will autolyzed. The autolyzed ofAcaudina Molopadioides will produce a lot of inconvenience to further enhance theirvalue. If we can study of autolysis of Acaudina Molopadioides, and try to use theAcaudina Molopadioides autolysis, we can completely make the AcaudinaMolopadioides autolysis into an advantage. This study shows thorough researching thebody wall autolysis of Acaudina Molopadioides, and optimizing the optimal autolysisconditions. On this foundation of Acaudina Molopadioides autolysis and exogenousenzyme preparation of active peptide collaborative process are studied, for thepreparation and utilization of Acaudina Molopadioides autolysis AcaudinaMolopadioides active peptide provides an important theoretical basis, for thecoordination with exogenous enzyme Acaudina Molopadioides autolysis of AcaudinaMolopadioides of the preparation of active peptide has higher nutrition and medicinalvalue. This study has paved the way for the high value of Acaudina Molopadioides use.The Acaudina Molopadioides body wall of protein content is much. Fat content isless.7kinds of essential amino acids contains very much. Acaudina Molopadioidesbody wall also contains complete kinds, abundant mineral elements. Examine the mainchemical composition of Acaudina Molopadioides body wall dissolution. Using thesoluble oligopeptide dissolution rate is as the evaluation index of the AcaudinaMolopadioides autolysis.Study indicates that Acaudina Molopadioides body wall has nothing to do withmicrobes. Under25℃or45℃environment, Acaudina Molopadioides place shouldnot exceed30hours. A slight variation of the improvement is little in the4hours ofautolyzed.Various factors influence on Acaudina Molopadioides soluble oligopeptidedissolution rate is in turn, pH>temperature>substrate concentration> NaCl concentration.Acaudina Molopadioides body wall autolysis conditions for optimum is that pH6.2,43℃,38%substrate concentration and the concentration of NaCl is26mg/mL,autolysis4h. Under the condition of the optimal autolysis of Acaudina Molopadioidesbody wall soluble oligopeptide content is12.25mg/g. On the basis of single factor experiment conclusion, through the response surfaceanalysis to optimize the optimal hydrolysis process, the best condition is that35%substrate concentration,0.112mkat/g enzyme dosage and45℃, hydrolysis4h. Thehydrolysate by ultrafiltration membrane of10000intercept molecular weight optimumis purification A. The nanofiltration membrane B is best for enrichment anddesalination.The Acaudina Molopadioides active peptide has a higher nutritional value. TheAcaudina Molopadioides active peptide has a wide range of food to add. The AcaudinaMolopadioides active peptide has certain antioxidant capacity.