Exploitation Of Medicinal Poly(Aspartate)Materials

Thermo-responsive polymers are a class of smart materials that have the ability to respond to achange of temperature. At present, thermo-responsive polymeric materials have been widely used forcontrolled drug delivery, biological separation, tissue engineering and bio-pharmaceutical products inthe biomedical fields. Therefore, good biodegradability and biocompatibility of the polymericmaterial is the objective requirements in the biomedical field, The preparation and characterization ofthis material is undoubtedly one of the most important tasks. The main content of this paper isdivided into three parts:First, the literature review section, a brief introduction to the types of smart materials, thepreparation method of poly(aspartate) materials and its practical application in the biomedical fields.Second, A new thermo-sensitive, biocompatible poly(N-propionyl-L-aspartic acid/ethyleneglycol)(PPAE) with the upper critical solution temperature (UCST) was synthesized bypolycondensation of L-aspartic acid and ethylene glycol. The chemical structures of the monomerand polymer have been confirmed by FTIR,1H NMR and elemental analysis measurements. Theturbidimetric measurement has indicated that PPAE showed the reversible UCST phase transition at1.5-37.6oC in pure water or the alcohol/water mixture. The molecular weight, molecular weightdistribution of PPAE, and concentration also played an important influence on the phase transition.UCST can be facilely tuned via changing the content of alcohol in water. Besides, the survival rate ofHela cells to PPAE is close to100%within48h, implying its excellent bio-compatibility. Suchtunable aspartic acid-based polymers with thermo-sensitivity and biocompatibility could be useful ina variety of the biomedical field.Third, using L-aspartic acid, butylene glycol, carboxylic acid as starting material,N-formyl-L-aspartic anhydride, N-formyl-L-aspartic acid, N-formyl-L-aspartic acid chloride wereprepared, the further preparation of the temperature-responsive poly(N-propionyl-L-aspartic acid/butanediol) material was performed. By infrared spectroscopy, nuclear magnetic resonance spectroscopy techniques, the intermediates, monomers and polymers chemical structure werecharacterized. UV spectral data showed that poly(N-propionyl-L-aspartic acid/butanediol) have anupper critical solution temperature (UCST), and the UCST is20℃. In addition, thetemperature-sensitive polymer material has excellent biocompatibility, the polymeric materials onHela cell survival rate reached up to100%within48hours. Obviously, good biocompatibility andthermal sensitivity of such polymeric materials provides good performance and security in thebiomedical application field.