| Silk peptide is the hydrolysis product of silk fibroin. Owing to its excellentbiocompatibility, antioxidation and enough amino acids, Silk peptide are widely usedin food, biomedical material, cosmetic, and textile industries. Glucose is the energymaterial of the life activities. It is very important for the determination of glucoseas a result of its biological energy source and metabolic intermediate properties. In thepaper, silk peptide and dyes system, the effects of foreign substances in silk peptideand dye system, dye and glucose system, and polymerization dye are investigatedwith spectroscopy and electrochemical methods. The all chapters of the paper aresummarized and presented as follows:1. A simple, fast and sensitive fluorescence method has been developed andvalidated for determination of glucose. CA had one strong fluorescence peak at586nm in PBS(pH7.0) buffer solution. After the addition of glucose into CA solution,the intensity of fluorescence peak at586nm for CA increased greatly. Moreover, theconcentrations of glucose and the difference of intensity showed a good linearrelationship over the range of2.0×10-7to1.0×10-6mol·L-1. The detection limit of themethod was1.22×10-6mol·L-1.The relative standard deviation was3.10%for fiveindependent determinations and3.48%for five successive determinations at6.0×10-7mol·L-1level. The common foreign substances in calcium gluconate tabletssuch as Ca2+and startch did not interfer determination. This method was used todetermine glucose in calcium gluconate tablets samples with92.51-101.85％recovery. Compared with the conventional O-Toluidine method, the results were inagreement.2. In this paper, the interaction of reactive brilliant orange K-7R(ROKR) with silkpeptide was studied by means of multiple spectroscopy(Ultraviolet visible,fluorescence spectroscopy, synchronous fluorescence, Rayleigh resonance spectrum and so on). The intrinsic fluorescence of silk peptide has been quenched by the staticquenching of reactive brilliant orange K-7R within a range from1.0×10-6to2.0×10-5mol·L-1.A new complex between SP and ROKR was formed. The bindingdistance and binding ratio were calculated as3.46nm and1:1, respectively.Furthermore，the conformational structure of the peptide chain became compressiveas result of changes in the region of tyrosine and tryptophan residues of silk peptide.Additionally, the effects of foreign substances in dyeing system such as surfactants,ionic liquids, metal ions, oxidants and textile pulp) on SP-ROKR were alsoinvestigated. The results showed that the binding constants increased when ionicliquids including chloride hydroxyethyl trimethyl amine, bromide1-ethyl-2,3–dimethyl imidazolium,1-ethyl-2,3-methyl imidazoliumbis-[trifluoromethyl-sulfonyl] imide, textile pulp (original starch, polypropylenealdehyde amine, aldehyde acid ester starch, oxidized starch), metal ions (Ca2+, Pb2+,Ba2+), and surfactants CTAB were added into SP-ROKR. However, the bindingconstants decreased when phosphate starch, Triton X-100, Cu2+and oxidants (30%H2O2, K2S2O8, K2Cr2O7) were in the presence of SP-ROKR, and on effect in thepresence of sodium dodecyl benzene sulfonate.3. The reactive brilliant orange K-7R as monomer was polymerized to form polyreactive brilliant orange K-7R film by the using of cyclic voltammetry method inpencil graphite. A poly reactive brilliant orange K-7R film pH sensor has beendeveloped using a novel electrochemical method-zero current potentiometry system.The poly reactive brilliant orange K-7R film coated pencil graphite electrode isconnected in series between the working and counter electrodes of a potentiostat, andimmersed in solution together with a reference electrode. When the solution pH varies,the resulting zero current potentiometry is linear with the values of the solution pH inthe range of1.81to9.37. The relationship of Ezcp(zero current potentiometry) and pHwas nernst response, and the response slope for-2.1mV/pH. The pH sensor showshigh stability and reproductive. Sometime, the determination of the pH of the samplesolution(PBS, CH3COOH/CH3COONa, disodium hydrogen phosphate-citric acidbuffer solution) was investigated the using of the poly reactive brilliant orange K-7Rfilm by zero current potentiometry. A relative error was within±5%. The proposedmethod was applied to determinate the pH with satisfied results.4. The interaction of reactive dark blue M-2GE(RDBMG) with silk peptide wasstudied by means of multiple spectroscopy. The intrinsic fluorescence of silk peptide has been quenched by the static quenching of reactive dark blue M-2GE within a range from1.0×10-6to1.2×10-5mol·L-1.A new complex between SP and RDBMG was formed, which was bounded by hydrogen bonding and van der Waals. The binding distance and binding ratio were calculated as3.04nm and1:1, respectively. Furthermore, the conformational structure of the peptide chain became compressive as result of changes in the region of tyrosine and tryptophan residues of silk peptide. Additionally, the effects of foreign substances in dyeing system such as surfactants, ionic liquids, metal ions, oxidants, textile pulp, and seven kinds of amino acids (L-histidine, L-aspartic acid, L-lysine, L-arginine, L-methylthio amino acid, L-leucine and L-proline) on SP-RDBMG were also investigated. The results showed that the binding constants increased when Ionic liquids including chloride hydroxyethyl trimethyl amine, bromide1-ethyl-2,3-dimethyl imidazolium,1-ethyl-2,3-methyl imidazolium bis-[trifluoromethyl-sulfonyl] imide, textile pulp (original starch, phosphate starch, oxidized starch), metal ions (Ca2+, Zn2+, Ba2+), surfactants(CTAB, Triton X-100, sodium dodecyl benzene sulfonate) and seven types of amino acids were added into SP-RDBMG. However, the binding constants decreased when polypropylene aldehyde amine, aldehyde ester starch, Cu2+and oxidants (30%H2O2, K2S2O8, K2Cr207) were in the presence of SP-RDBMG.5. Silk peptide can been absorped on the surface of pencil graphite by the using of automatic adsorption method to form silk peptide film as SP/PR electrode, The dynamics equation of the interaction of silk peptide and reactive dark blue M-2GE was investigated by zero current potentiometry, which was applied to explore kinetic parameters. It found that the silk peptide binding with reactive dark blue M-2GE accorded with first order kinetics equation, which was1n(1-τi)=-k[EtOH]0ti among1-τi represents (Ezcp,τi-Ezcp,τ＝0-Ezcp,τ=1).And the chemical reaction rate constant and half-life were also obtained, k was4.05×103S-1, t1/2was1.71×10-4s respectively. SP can combine with M-2GE, forming a complex(SP-RDBMG) with binding ratio1:1.12and apparent binding constant α=1.12×106... |
PDF Full Text Request