The Application Of Octa-carboxylic Pthalocyanine Aluminum In Fluorescence Analysis

Pathalocyanines are a category of compounds which are similar to that ofporphyrins in structure. Phthalocyanine and metal phthalocyanine have been widelyapplied in catalysis, organic semiconductors, light conductor, color photography, laser,Liquid crystal display (LCD), Langmiur-Blodgett (LB) films, photodynamic therapydue to their displays extraordinarily high photostabilities and thermal and chemicalstabilities. The water-soluble metal phthalocyanines possess high extinctioncoefficients and favorable fluorescence quantum yields. Moreover, fluorescenceemission of the water-soluble in the red or near-infrared region, which means greatdecrease of interference from background fluorescence and scattering lighting.Focusing on synthesis of the octa-carboxylic pthalocyanine aluminum andconstruction of novel analytical method base on properties of fluorescencespectroscopic of the water-soluble. This thesis carried out some research as follows.1. The microwave-assisted synthesis and characterization of octa-carboxylicpthalocyanine aluminumOcta-acyl amino phthalocyanine aluminum was synthesized by microwaveirradiation and using Pyromellitic anhydride, urea and anhydrous aluminum chlorideas raw materials, and ammonium molybdate as catalyst. Then, octa-carboxylicpthalocyanine aluminum was synthesized by alkaline hydrolysis reaction. Theinfluence of conditions of pyromellitic anhydride and urea molar ratio, microwavepower, reaction time and the dosage of the catalyst were invested. The structures ofthe products were characterized by IR, UV and fluorescence analysis. The results ofexperiment show that the microwave synthesis of phthalocyanine has not only fasterreaction time but less pollution and higher purity compare with traditional liquidmethods. But the production rate is lower.2. Research of the interaction between octa-carboxylic pthalocyanine aluminum(AlC8Pc) and bovine serum albumin (BSA) and detection of human serumalbumin by fluorescence spectroscopyThe interaction between octa-carboxylic pthalocyanine aluminum (AlC8Pc) and bovine serum albumin (BSA) was studied by fluorescence spectroscopy and UV visabsorbance. The stern–volmer quenching constant Ksv, thermodynamic parametersand the binding site number were calculated at different temperatures. The resultdemonstrated that the mechanism of quenching belongs to static quenching and themainly binding force is due to Van der Waals interactions or hydrogen bonds. Thisfluorescence quenching was proportional to the concentration of serum albumin, thuscould be used for near-infrared fluorescence sensing of serum albumin. Under theoptimum conditions, the fluorescence quenching showed a good linear relationshipwith the concentration of serum albumin in a wide dynamic range from6.7×10-9to1.3×10-7mol·L-1with a detection limit of2.0×10-9mol·L-1(S/N=3). The proposedexhibited acceptable stability and reproducibility and was successfully applied todetermine human serum albumin in serum samples with satisfying results.3. Determination of Cu2+based on near-infrared fluorochrome (NIRF) ofocta-carboxylic phthalocyanine aluminumThe molecular spectroscopy properties of octa-carboxylic phthalocyaninealuminum (AlC8Pc) were studied. A novel method of fluorescence quenching for thedetermination of Cu2+based on near-infrared fluorochrome (NIRF) of AlC8Pc (themaximum excitation and emission wavelengths at624nm and698nm) wasestablished. The effect factors were optimized. Under the optimum conditions,(pH4.5, AlC8Pc concentration of5.0×10-6mol·L-1, reaction time of5min and reactiontemperature at room temperature) the linear range of the calibration curve for thedetermination of Cu2+is from2.5×10-6~3.0×10-5mol·L-1, with a correlation coefficient(R) of0.9974. The limit of detection is8.3×10-7mol·L-1. The method was applied inthe determination of Cu2+in real water samples.4. Determination of gentamicin using octa-carboxylic phthalocyanine aluminumin gentamicin injections by fluorescence spectroscopyBase on the fluorescence spectroscopy properties of octa-carboxylic phthalocyaninealuminum, a novel of fluorescence method for the determination of gentamicin wasestablished. The effect factors were optimized. Under the optimum conditions,(pH3.0, AlC8Pc concentration of4.5μg·mL-1, reaction time of10min) the linear range of the calibration curve for the determination of gentamicin is from9.6×10-8~6.7×10-7g·mL-1and6.7×10-7~1.3×10-6g·mL-1, with a correlation coefficient (R) of0.9945and0.9986. The detection limit of the method is0.023μg·mL-1; the method was appliedfor the determination of gentamicin injections with satisfactory results.