Contrastive Study On The Condition Optimization Of Cellulase Xtraction In Different Reverse Micelles

Cellulose is an abundant natural resource. However, it is usually used in a simpleway such as incineration, which not only a waste of resources but also cause pollution.The biotechnology degradation of cellulose is an effective way, and exploration ofefficient lignocellulosic enzymes is of great practical significance.The theory of Reverse micelles extraction is as follows:The reverse micelle which contains enzyme protein is formed in the two-phaseinterface due to the interaction between the surface of the reverse micelle solution andthe surface of the enzyme protein in the two-phase interface after the contact ofreverse micelle solution and the enzyme protein-containing aqueous phase. Theextraction of the enzyme protein is achieved when reverse micelle diffusion into theorganic phase. Reverse micelles extraction technique has many advantages such aslow costs, repeatedly use of the solvent, mild extraction rate and conditions, not easyto cause the denaturation of enzyme protein.At present, domestic and foreign research are focused on the type of reversemicelle system which based on chemical surfactant. This type of reverse micellesystem has many limits such as limited resources and environmental pollution.Biosurfactant attracts people’s attention for its unique structure, environment,non-toxic, bio-degradable, etc. Currently, the contrastive study on the conditionoptimization of cellulase extraction in different reverse micelles using biosurfactantsand chemical surfactants is poorly.The purpose of this study is extraction and separation of cellulase effectively. The extraction conditions and mechanism of the reverse micelle system built on threekinds of surfactants (AOT, CTAB, and RL) were investigated. The effect factors werefocused on aqueous phase pH, ionic strength, ionic species, the kind of the surfaceactive agent and concentration. Construction of anti-micellar extraction conditionsand mechanism of the system. This paper comparatively studied the optimalextraction conditions of cellulase through different reverse micelles constructed ba sedon cationic surfactant CTAB, anionic surfactant AOT and RL, respectively. Severalimportant parameters affecting the cellulase extraction process were investigated,such as aqueous pH, surfactant concentration, ionic strength and salt species. It wasshown that the optimal pH value of aqueous phase in both AOT and RL reversemicelles (anionic surfactant) was3.0, while in cationic surfactant CTAB reversemicelle the optimal result was obtained at8.0. The optimal surfactant concentrationswere23.7mmol/L,15mmol/L and2.75mmol/L for AOT, CTAB and RL, respectively.For all three reverse micelles, the forward extraction of cellulase peaked at0.05mol/LNaCl in the aqueous phase. Compared with chemical surfactant, there is no distinctadvantage observed for the reverse micelles formed based on bio-surfactant. However,the bio-surfactants have a unique biological nature. Instead of chemical surfactants,they are likely to play a critical role in the protein extraction and its purification in thefuture.