Study Of Phycocyanin Extracted From Spirulina Platensis Using Reverse Micellar Hydrated Extration Technology

Phycocyanin, as one of the main components in Spirulina platensis, has been applied in food, cosmetic and medicine industries, etcSome important physiological activities such as antioxidant,antitumor and antiviral effect are generally identified,thus phycocyanin has wider application prospect.The new and innovative separation technology named as"reverse micellar hydrated extraction technology", which integrated hydrate separation with reverse micellar extraction, is used for the control of biological activity and extraction of bio-product. The kinetic research was implemented on the extraction of phycocyanin from the reverse micellar system, which was formed by CTAB,n-octane and n-pentanol. Correspondingly,the extraction behavior of phycocyanin from the reverse micellar system as well the effect mechanism of temperature,pressure,CTAB concentration,and initial water content W0 on phycocyanin extraction were acquired. The results contributed new information for the further research on the control of biological activity and reverse micellar hydrated extraction of bio-product.Contrast experiments of ethylene hydrate formation in pure water system and reverse micellar system showed that the kinetics of hydrate formation had large difference between two systems,which was caused by the different solubility,dissolution rate of ethylene and the water content of two systems.The reverse micellar hydrated extraction experiments showed that, kinetic curves of ethylene hydrate formation in the reverse micellar system containing phycocyanin were similar to those of the reverse micellar system not contained phycocyanin. The maximum extraction yield of 81.3 % was obtained at initial temperature of 3.0℃, initial pressure of 4.0 MPa, CTAB concentration of 0.05 mol/L and initial water content of 40. Because of the large gas liquid ratio for the reverse micellar hydrated extraction experiments,the initial temperature and pressure were non-significant factor effecting extraction yield. And the increased CTAB concentration and initial water content favored the extraction yield of phycocyanin.