Production Of Bacillus Amyloliquefaciens Using Citric Acid And Potato Starch Wastewater For Improving Vegetables Growth And Inhibiting Cyanobacteria

Organic wastewater have high COD concentration and huge emission every year. It willpollute the environment and consume large amounts of energy with some treatments. Organicwastewater contains a great deal of material can offer the microorganism for growth. Bacillusamyloliquefaciens adapt to the environment speed, has the ability to inhibit a variety ofpathogenic bacteria and promote the role of plant growth. This paper is mainly on theoptimization of Bacillus amyloliquefaciens using citric acid and potato starch wastewater forapplication. In order to make the most use of wastewater, offering the referred data andexplore the application of the Bacillus amyloliquefaciens. Main research results are asfollows:1The optimization of Bacillus amyloliquefaciens using citric acid wastewater make themaximum yield of2.1×10~9CFU/mL in21h at31°C, pH8.0, no nitrogen and any things.2The maximum yield of2.2×10~9CFU/mL of Bacillus amyloliquefaciens was reached in24h at36°C, pH7.5using the potato starch wastewater as substrate.3With100times diluted solution of citric acid wastewater of fermentation of Bacillusamyloliquefaciens soak Chinese mustard seed and increase germination rate by44%in thedish,20~25℃. Chinese mustard is cultivated with pot in the greenhouse. Use the urea in thethirty-sixth and then use the fermentation of Bacillus amyloliquefaciens. There is obviouslylower in the effect of the nitrate and nitrite content on Chinese mustard, have significantlyreduced role with the blank control group, the plant height, plant weight and vitamin C withno adverse effects. Using100mL diluted100times of citric acid wastewater of fermentationbacteria water the Chinese mustard, had no adverse effect on rhizosphere microbes in soiland inhibition the fugus of the pathogenic bacteria. Fermentation liquor can be used as abiological fertilizer in the future.4When the concentration of the Cyanobacteria is lower, have the stronger inhibitionwith the use of larger doses the citric acid wastewater of fermentation of Bacillusamyloliquefaciens.