Preparation Of Dextrin Nanosphere With Inverse Microemulsion

As a type of medicine carriers, nanospheres could be enzymolysised by homologousenzyme，and that could be prepared as targeting preparation by covalence cross-linked withantibody，to enhigh the content of preparation in focus of infection and to belower adversereaction. For these reasons nanospheres have wide application outlook in medicament field.Scientists prefer to take natural polymer as the raw material to prepare nanospheres andthe preparation of polysaccharides nanospheres is the one which many scientists focus on.There are many methods for their preparation, of which inverse microemulsion is the onescientis focus on, W/O microemulsion is constituted by oil phase, aqueous phase andsurfactant. The inverse microemulsion were prepared from N-hexane as oil phase, deionizedwater as aqueous phase, Span60and Tween60as surfactant，N-pentanol as cosurfactant. Theeffect of factors in inverse microemulsion was analysised. The dextrin nanospheres weresynthesized from dextrin as material, Na3P3O9as crosslinking agent.Adjust the amount of each reagent to satisfy the requirement of reaction environmentbefore preparation dextrin nanosphere. The cross linking reaction was detected by Infra-redspectrum analysis. And the dextrin nanosphere was measured with a SEM.The results were showed: In the inverse microemulsion system, S/O was norientationcorrelated with W/O at certain range，while A/O was antidromic；Raise the concentration ofNaOH will lower the dissolved water capability of microemulsion，to solve this problem wecan raise the HLB value to compensate the loss dissolved water capability of microemulsion.The eligible conditions were as follows: N-hexane, dextrin solution, Span60, Tween60,N-pentanol were100mL,30mL,6.6g,13.2g,16mL respectively. The microemulsion was highclarity and good liquidity；The cross linking reaction taking place successfully was detectedby Infra-red spectrum analysis. A uniform dextrin nano-microsphere with a diameter500nm~600nm was measured with a scanning electronic microscope.