Facile Method To Engineer Pluronic F127Functionalized Magnetite/Graphene Nanohybrid For Chemophototherapy

In this study, a multifunctional graphene-based nanohybrid, termed as GN/Fe3O4/PF127, was engineered via a one-pot process including the simultaneous reduction of graphene oxide/Fe3O4and assembly of Pluronic F127(PF127) onto graphene nanosheets (GN) via a facile way. GN with the aromatic and planar structure supports the attachment of multiple functional components including MRI contrast agent (Fe3O4nanoparticle) and the aromatic anticancer drug like doxorubicin (DOX), as well as PF127coating that enables the nanohybrid with both high dispersivity and stability in physiological environment. The successful assembly process was revealed by TEM observation, size and FITR monitoring. In contrast with the individual graphene or its oxide derivative, the resulted GN/Fe3O4/PF127nanohybrids show high biological dispersion and MRI effect for diagnosis due to the incorporation of super paramagnetic Fe3O4nanoparticle without evident cytotoxicity. Moreover, GN exhibits high optical absorption in the near-infrared region, affording GN/Fe3O4/PF127nanohybrid with the photo-thermal effect. In combinational with the chemotherapy effect of DOX, GN/Fe3O4/PF127nanohybrid can be an ideal platform for chemophototherapy. Cellular toxicity assays indicate DOX loaded GN/Fe3O4/PF127nanohybrid show a remarkable cytotoxicity to the HeLa cells and the cytoxic effect was intensified when subjected to photo irradiation. Confocal laser scanning microscopy (CLSM) and flow cytometric analysis (FCAS) revealed that the nanohybrid can be easily uptaken into HeLa cells.