Determination Of Phenol And Tsumacide By Sequential Injection Analysis With Chemiluminescence Detection

In this dissertation, the sequential injection coupled with chemiluminescence detection technique was proposed and applied to the determination of phenol and tsumacide and satisfactory results were obtained.The first section of the dissertation introduced the concept, classification, origin, harm of phenol and the review on the progress of analytical methods for the determination of phenol in recent years. The methods including spectrophotometry, brominating volumetric method, fluorescence analysis, electrochemistry, chromatography and chemiluminescence were evaluated in detail.The second section of the dissertation developed a sequential injection analytical method with chemiluminescence detection for the determination of total phenol in the atmosphere and volatile phenol in water samples. In the acidic medium, phenol can react with potassium permanganate and produce chemiluminescence, which is greatly enhanced by formaldehyde. The chemiluminescence intensity is linearly related to the concentration of phenol in the range0.005-0.8mg/L with a correlation coefficient of0.9940. The relative standard deviation is0.65%for0.2mg/L phenol solution in11repeated measurements. The sampling frequency is146samples per hour. The detection limit (3σ) is0.003mg/L. The percentage of recovery for the determination of phenol in environmental samples is over90.0-110%. The result on the determining of phenol in a NBS standard reference material showed very agreement with the certified value.The third section of the dissertation proposed a sequential injection analysis combined with chemiluminescence to determine tsumacide in vegetables. In the nitric acid, the alkaline degradation product of tsumacide can react with potassium permanganate when rhodamine6G is present. Within a certain range, the chemiluminescence intensity is linearly related to the concentration of tsumacide. Based on this, a sequential injection chemiluminescence method to determine tsumacide pesticide residue is set up. The chemiluminescence intensity is proportional to the concentration of tsumacide in the range from0.002to0.15mg/L. The detection limit (3a) is0.0009mg/L and the relative standard deviation for thirteen repeated measurements for0.1mg/L tsumacide is1.5%. The sampling frequency is145samples per hour. The method was applied to the determination of tsumacide in rape, cabbage, lettuce and spinach is over96.7-110%.In the last section, a summary for the characteristic of sequential injection coupled with chemiluminescence detection technique was described.