Preparation Of Cellulose Beads With Ionic Liquid Mixed Solvent And Its Application

Chromatography has been applied to the separation of various biochemical products, especially active macromolecules, which shows some advantages such as mild conditions, high efficiency and selectivity. The characteristic of the chromatographic media is key factor to affecting the performance of chromatography, so the excellent media has been one of the focuses of chromatographic research. In this paper, microcrystalline cellulose (MCC) was used as raw material. The solubility properties of MMC in new ionic liquid mixed solvent were studied. The method of water-in-oil suspension regeneration was applied to prepare cellulose porous beads. After coupling functional groups, the adsorption properties of the media were investigated. Main results are as following.1. MMC was directly dissolved in novel1-butyl-3-methylimidazolium chloride (BmimCl)/1,3-dimethyl-2-imidazolidinone (DMI) mixed solvent. Some factors including the mixing ratio of the solvent and dissolving temperature were studied to optimize the preparation of cellulose viscose. The mechanism of cellulose dissolution was preliminary explained by analyzing the structure changes of cellulose before and after regeneration.2. The prepared cellulose viscose was used to produce four kinds of cellulose porous beads, i.e. Cell1, Cell-2, Cell-3, Cell-4, with the method of reversed-phase suspension regeneration. Some physical propeties were investigated, including wet density, water content, pore volume, porosity, specific surface area, average pore size, pore size distribution, particle size distribution and appearance. Based on these results, Cell-1was selected to further prepare the chromatographic media due to good sphericity, large specific surface area and appropriate pore distribution.3. Cell-1beads were used as the matrix to couple anion-exchange ligand DEAE and hydrophobic charge induction chromatographix ligand MMI. The ion-exchange capacitiy was about450μmol/ml, and the HCIC ligand density was81.3μmol/mL. BSA and IgY were used as target protein to determine the adsorption properties of two media. The results showed that both media have high adsorption capacities and strong interactions with proteins, which had a potential as chromatographic media for protein separation.