Studies On The Separation Of Anaerobic And Its Interaction With Sulfate Minerals From Mixed Area Of Landfill And Tailings In Xiangshan

The Xiangshan Landfill is constructed on the iron sulfides tailings, mixed withporphyrite iron ore tailings and acid mineral drainage in Ma’anshan City. The landfillwaste from this landfills a complex mixture which consist of organic waste, sulfates,iron oxide waste，microbial electron acceptor and donor. According to the previousresearch, the interaction of microbial growth metabolism with microorganism-mineralof pyrite tailings has great impact on the environmental cycle of. C, N, S and someheavy metal elements. In order to study the characteristics of anaerobic bacteria andits environmental effect, physiological and biochemical characteristics of the isolatedpure bacteria were discussed in this paper, as well as its interaction with minerals.Detailed results are given as follows:A strain of gram-negative bacterium was found,spherical, and belongs to thebacterium (Stenotrophomonas sp.) based on the result of16SrDNA sequencealignment. Literature shows that this bacterium widely exist in nature is a kind ofconditional pathogenic bacteria, and causing infection as a sojourner in person’srespiratory and intestinal tract.Another strain is one of the gram-negative bacterium, which belongs tofusiformis with inflated circular and regular rod at either end.16SrDNA sequencealignment and functional testing shown the bacterial strain was identified asClostridium Sulfate Reducing Bacteria(abbreviated as C.SRB). The results of thephysiological-biochemical characteristic of C.SRB showed that this bacterium can useglucose and lactose as carbon source; methyl red test showed negative; indole test andcatalase test both showed positive. It was found the highest consumption ratio rate ofthe sulfate was0.73mmol·g/h when C.SRB was cultivated for36h; and the optimumgrowth temperature, pH and salinity were35oC7and0.5g/L respectively.Comparative experiments were designed to research the interactions of threekinds of SRB (C.SRB, Desulfovibrio sp., and SRB-ATCC29577) with gypsum andanhydrite. The results found the three SRBs can reduce gypsum and anhydrite to H2Sand dissolved sulfide, respectively. The C.SRB produced the largest amount of H2S and dissolved sulfide SRB-ATCC29577ranked second. While Desulfovibrio sp.produced the least amount of H2S and dissolved sulfide.