Construction Of The Self-assembly Of Octenylsuccinic Corn Dextrin And Its Application In Curcumin Solubilization

Synthetic (polyester, polyether and lactic acid copolymer) and natural (protein and so on) amphiphilic polymers, can self-assembly in water to form nanoscale (10-1000nm) micelles and vesicles by hydrophobic interaction. The self-aggregate structure formed by amphiphilic polymers can load hydrophobic drugs and nutrients, and play a role in controlled-release and target transport in human body (in vivo). Thus, the amphiphilic polymer has gradually become a research hotspot in medicine, chemical and food industry in recently years.Dextrin is a sort of biological macromolecular, which has a wide range of applications in food industry, such as adipose substitute, thickener and auxiliary material in spray drying. But up to now there have been little research on hydrophobic modified products of dextrin and its application in domestic and other countries. In this paper, we chose dextrin as the research subject, and use ocentyl succinic anhydride (OSA) as the modifier to obtain octenylsuccinic corn dextrin ester (OSCDE). In optimizing the condition of forming self-assemblies, this article studied its solubilization effect on curcumin. The research results are as follows:1. The fluorescence spectrometry, dynamic light scattering (DLS) were used to find that beyond the critical aggregation concentration (CAC), OSCDE produced spherical self-aggregates in aqueous solution. The microspheres were observed by transmission electron microscope (TEM) to be monodispersed sphere or spheroidicity and with the diameter around200nm.2. The CAC, nanoparticles size and the absolute value of zeta potential were all affected by the structure of OSCDE. The higher the degree of substitution (DS) the lower the CAC value and size of the nanoparticles, while the higher the absolute value of zeta potential. Increase in molecular weight (Mw) definitively increased the CAC, size and absolute value of zeta potential. The effects of environment parameters on self-assembly behavior mainly reflected as follows. Reduce the pH of the solvent system, increase the ionic strength or rise up the temperature can result in lower absolute value of zeta potential, larger nanoparticle size and wider distribution. However, modified the sample concentration could not induce the change of nanoparticle size, distribution and the absolute value of zeta potential.3. This article chose DS of OSCDE, molecular weight (Mw) of dextrin and the stirring power as the single factor. Response Surface Analysis (RSA) was applied to optimize the solubilization of curcumin. The regression equation is as follows: Y=4.44132+0.54250*X1+0.23250*X2+0.32500*X3+0.095000*X1*X2-0.31500*X1*X3+0.085000*X2*X3-1.10316*X,2-1.02816*X22-0.96816*X32Work out the optimum craft parameters:DS0.0302, Mw10.4×104g/mol, stirring power4.0w. Under the optimal parameters we got the dispersion of curcumin at4.44μg/mLo4. The load experiments indicated that the nano-particle size increased after solubilization of curcumin, the uniformity declined and self-assemblies became instability after coating of curcumin. The redissolving experiments indicated that the distribution became widen while the particle size and zeta potential had no significant variation. This article studied the structural characterization of the OSCDE loaded with curcumin. The fourier transform-infrared spectroscopy (FT-IR) indicated that the curcumin has an obvious hydroxyl absorption peak around3490cm-1. Similarly, a weak peak at1465cm-1has observed in all the three cases could be due to-CH2bending vibration. The signature peaks at1627cm-1and1602cm-1are found in native curcumin and nanoparticulate curcumin were due to C=O double bonds and aromatic C=C double bonds respectively. The compound of curcumin and OSCDE has an obvious absorption peak at1500cm-1to1600cm-1because of the benzene ring skeleton, while the OSCDE loaded with curcumin didn’t have this characteristic peak. X-ray differaction (XRD) indicated that curcumin had many significant diffraction peaks, while OSCDE loaded with curcumin had no characteristic peak. Differential scanning calorimetry (DSC) indicated curcumin had one decalescence peak at180℃which OSCDE loaded with curcumin didn’t have this decalescence peak.