Based On The Biological Parameters On-line Detection Of Glutamic Acid Fermentation And Its Dynamics Research

In the use of glutamic acid rods coli (Corynebacterium gulutamicum) S9114fermentation in the production of the industrialized production of glutamate, nobiomass on-line inspection report. In order to solve this problem, this paper developedby use of shandong academy of sciences institute of SBA-60series biomass on-linedetection system for glutamate fermentation biological parameters online real-timedetection, and on the basis of glutamic acid fermentation dynamics research, the mainwork and research results were as follows:1. This article through to glutamic acid rods coli (Corynebacterium gulutamicum)S9114ultraviolet mutagenesis, nitrite mutagenesis and hybrid mutation, final yield ofglutamic acid strain, acid yield up to10.0%.2. Taking glutamic acid fermentation as the research object, using the developedby shandong academy of sciences institute of SBA-60series biomass on-linedetection system of glutamic acid fermentation organisms parameter contents ofglucose, lactic acid, glutamate content, real-time detection, OD value has greatsignificance.3. In this paper, the experimental data using the MATLAB software to carry onthe fitting, eventually adopted by the bacteria growth dynamics of the model of theLogistic model, product generation and bacteria growth part of the coupling of theproduct to generate dynamic model, the substrate is mainly used for cell growth andmetabolites of synthetic substrate consumption have been building a successful anddynamic model and the fitting effect is very good.Computer instead of manual detection and control of the fermentation process isthe development direction of modern biological engineering, has certain theoreticalresearch foundation, but at this stage the fermentation process control the widespreadpresence of a technical problem that is not stable and reliable bio-sensor analyticalinstruments, leaving many fermentation control cannot reach expected, unable toreal-time detection of each related state variable and the biochemical indicators. Theproject in combination with parameters such as temperature, PH, dissolved oxygen,mainly according to glutamic acid, glucose, lactic acid and other parameters, explorevarious substrate fermentation environment reduce the associated trends and productform, set the related parameters for fermentation kinetics model of the independent variables, to the amino acid fermentation control optimization provides practicaltheory support.