The Research Of Fermentation Process For Cephamycin C

In this article one high-production strain was obtained after selection of spontaneous mutation and UV-induced mutation from the original strain Streptomyces sp. OFR1022producing cephamycin C, which made the fermentation potency of cephamycin C be1.74times as much as the original.Firstly, the spore slants with more plentiful spores, darker color and shortened mature cycle were obtained after the optimization of spore slant medium and culture conditions of the high-production strain. Subsequently, the ratio of delayed carbon and nitrogen sources was optimized by Box-Behken design for response surface methodology(RSM), and the additions of corn starch(58.73g/L), cottonseed meal(19.65g/L) and ordinary fish meal(12.49g/L) could make the fermentation potency of cephamycin C increased by40.0%. Meanwhile the fermentation potency of cephamycin C was found to be increased obviously by the additions of growth factor, sulfur sources and amino acids in the fermentation medium. When0.6g/L biotin B7was added in the fermentation medium, a14.7%increase in the production of cephamycin C was observed. Besides that, sodium sulfite, potassium sulphate and sodium sulfate as sulfur sources were found to perform an positive role in promoting cephamycin C synthesis, while the addition of0.6g/L sodium sulfate could result in a9.1%increase in the production of cephamycin C. And through optimizing the additions of amino acids as precurors of cephamycin C synthesis by central composite rotatable design(CCRD) for RSM, the additions of L-lysine(0.3g/L), L-valine(0.2g/L), L-cysteine(0.15g/L) and DL-methionine(0.25g/L) were found to result in a27.2%increase in the production of cephamycin C.Finally, a7.1%increase for the fermentation condition optimization, in which the strain was cultivated in28℃for48h and sequentially in25℃till the end of the fermentation, was observed. Ultimately, the fermentation potency of cephamycin C was increased to3005.7μg/mL from the original900.8μg/mL through a series of optimizing tests.