The Prepration And Application Of The Boronate Affinity Materials Based On Polyacrylic Acid Microspheres

The pre-treatment of the sample is important when study the complex samples. The trace substance was enriched rapidly and efficiently can improve the sensitivity and accuracy of the determination. The biological substance that contains cis-diol compound is an important compound, including carbohydrates, glycoproteins, RNA and nucleosides. Because many cis-diol molecules of important biological are present in very low abundance in samples while interfering substance are usually present in high abundance, specific capture and effective enrichment of target cis-diol biomolecules become key, challenging steps in the study of the complex samples. The boric acid affinity materials have higher selectivity towards the cis-diol, and have been widely used in the biological molecules that contain cis-diol.In this study, we synthesized a new boronate affinity material, the polyacrylic acid microsphere contains amino group was used as support and applied it for the specific capture of the cis-diol molecules. The content including:1. This study synthesized a new boronate affinity material. The material chooses the polyacrylic acid microsphere contains amino group as the support and the4-Formylbenzoic acid as functional ligand, and the support was modified by the4-Formylbenzoic acid in "one pot" method. The results demonstrated that the boronate affinity material exhibited a high adsorption capacity toward cis-diol containing compounds (448μmol/g).2. The new boronate affinity material was used to concentrate quercetin of the red wine. At the same time, we studied the effect of the binding pH, the amount of adsorbent and volume of the elution solvent on the extraction efficiency of quercetin, and the experiment condition have been optimized. The results indicated that the quercetin can have highest extraction efficiency when the binding pH was7.0, the amount of adsorbent reach150mg and eluted the quercetin with5.0mL of0.1mol/L hydrochloric acid in methanol solution. Under the optimal extraction conditions the linear range of quercetin was0.4-2.8μg/mL, the detection limit was0.12μg/mL. The content of different red wines were detected and the content of quercetin was0.135μg/mL～0.280μg/mL.3. We explore the adsorption of cis-diol in the nonaqueous solvent. The result demonstrated that boronic acids can bind with hydrophobic compounds containing diol moieties in the organic solvent, and has a good selectivity. Besides, we found that the adsorption kinetics of various cis-diol is similar in different solvent system.